Saylor Timothy C, Savage Christina R, Krusenstjerna Andrew C, Jusufovic Nerina, Zückert Wolfram R, Brissette Catherine A, Motaleb Md, Schlax Paula J, Stevenson Brian
Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, KY, USA.
Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical Center, Kansas City, KS, USA.
bioRxiv. 2023 Feb 6:2023.02.06.527361. doi: 10.1101/2023.02.06.527361.
The SpoVG protein has previously been found to be a DNA- and RNA-binding protein. To aid in the elucidation of ligand motifs, affinities for numerous RNAs, ssDNAs, and dsDNAs were measured and compared. The loci used in the study were and , with particular focus on the untranslated 5' portion of the mRNAs. Performing binding and competition assays yielded that the 5' end of mRNA had the highest affinity while the lowest observed affinity was to the 5' end of mRNA. Mutagenesis studies of RNA and ssDNA sequences suggested that the formation of SpoVG-nucleic acid complexes are not entirely dependent on either sequence or structure. Additionally, exchanging uracil for thymine in ssDNAs did not affect protein-nucleic acid complex formation.
此前已发现SpoVG蛋白是一种DNA和RNA结合蛋白。为了有助于阐明配体基序，对其与多种RNA、单链DNA和双链DNA的亲和力进行了测量和比较。该研究中使用的基因座是[具体基因座未给出]，特别关注mRNA的5'非翻译区。进行结合和竞争试验得出，[具体mRNA名称未给出]mRNA的5'端具有最高亲和力，而观察到的最低亲和力是对[具体mRNA名称未给出]mRNA的5'端。对[具体RNA名称未给出]RNA和单链DNA序列的诱变研究表明，SpoVG-核酸复合物的形成并不完全依赖于序列或结构。此外，在单链DNA中将尿嘧啶替换为胸腺嘧啶并不影响蛋白质-核酸复合物的形成。
