Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.
Department of Biomedical Engineering, Washington University in St. Louis, Saint Louis, MO 63130, USA.
STAR Protoc. 2023 Mar 17;4(1):102132. doi: 10.1016/j.xpro.2023.102132. Epub 2023 Feb 24.
Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022)..
现有的聚焦超声（FUS）联合微泡介导的血脑屏障（BBB）开放（FUS-BBBO）的临床前研究方案需要昂贵的超声设备和复杂的操作程序。我们为临床前研究的小动物模型开发了一种低成本、易于使用和精确的 FUS 设备。在这里，我们提供了一个详细的协议，用于构建 FUS 换能器，将换能器连接到立体定向框架上以实现精确的大脑靶向，应用集成的 FUS 设备在小鼠中进行 FUS-BBBO，并评估 FUS-BBBO 的结果。有关此方案使用和执行的完整详细信息，请参见 Hu 等人（2022 年）。
