EMBL Australia Node in Single Molecule Science, Department of Molecular Medicine, School of Biomedical Sciences, The University of New South Wales, Sydney 2052, Australia.
J Cell Sci. 2023 Mar 1;136(5). doi: 10.1242/jcs.260749. Epub 2023 Mar 2.
Motor proteins are key players in exerting spatiotemporal control over the intracellular location of membrane-bound compartments, including endosomes containing cargo. In this Review, we focus on how motors and their cargo adaptors regulate positioning of cargoes from the earliest stages of endocytosis and through the two main intracellular itineraries: (1) degradation at the lysosome or (2) recycling back to the plasma membrane. In vitro and cellular (in vivo) studies on cargo transport thus far have typically focussed independently on either the motor proteins and adaptors, or membrane trafficking. Here, we will discuss recent studies to highlight what is known about the regulation of endosomal vesicle positioning and transport by motors and cargo adaptors. We also emphasise that in vitro and cellular studies are often performed at different scales, from single molecules to whole organelles, with the aim to provide a perspective on the unified principles of motor-driven cargo trafficking in living cells that can be learned from these differing scales.
马达蛋白是对细胞膜结合区室（包括含有货物的内体）的细胞内位置进行时空控制的关键因素。在这篇综述中，我们重点关注马达蛋白及其货物衔接器如何调节货物从内吞作用的最早阶段到两个主要的细胞内途径的定位：（1）在溶酶体中降解，或（2）回收回到质膜。迄今为止，关于货物运输的体外和细胞（体内）研究通常独立于马达蛋白和衔接器，或者膜运输。在这里，我们将讨论最近的研究，以强调马达蛋白和货物衔接器对内体囊泡定位和运输的调控。我们还强调，体外和细胞研究通常在不同的尺度上进行，从单个分子到整个细胞器，目的是从这些不同的尺度上提供对活细胞中马达驱动货物运输的统一原理的认识。
