谷胱甘肽 S-转移酶基因多样性及其在亚硝酸盐胁迫下中国绒螯蟹（Eriocheir sinensis）中 Nrf2 的调控。
Glutathione S-transferase gene diversity and their regulation by Nrf2 in Chinese mitten crab (Eriocheir sinensis) during nitrite stress.
机构信息
Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, China.
Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu Province 210023, China.
出版信息
Gene. 2023 May 15;864:147324. doi: 10.1016/j.gene.2023.147324. Epub 2023 Feb 28.
Eriocheir sinensis is one of the most important economic aquatic products in China. However, nitrite pollution has become a serious threat to the healthy culture of E. sinensis. Glutathione S-transferase (GST) is an important phase II detoxification enzyme, which plays a leading role in the cellular detoxification of exogenous substances. In this study, we obtained 15 GST genes (designated as EsGST1-15) from E. sinensis, and their expression and regulation in E. sinensis under nitrite stress were studied. EsGST1-15 belonged to different GST subclasses. EsGST1, EsGST2, EsGST3, EsGST4, and EsGST5 belonged to Delta-class GSTs; EsGST6 and EsGST7 are Theta-class GSTs; EsGST8 is a mGST-3-class GST; EsGST9 belonged to mGST-1-class GSTs; EsGST10 and EsGST11 belonged to Sigma-class GSTs; EsGST12, EsGST13, and EsGST14 are Mu-class GSTs; EsGST15 is a Kappa-class GST. Tissue distribution experiments showed that EsGSTs were widely distributed in all detected tissues. The expression level of EsGST1-15 was significantly increased in the hepatopancreas under nitrite stress, indicating that EsGSTs were involved in the detoxification of E. sinensis under nitrite stress. Nuclear factor-erythroid 2 related factor 2 (Nrf2) is a transcription factor that can activate the expression of detoxification enzyme. We detected the expression of EsGST1-15 after interfering with EsNrf2 in the hepatopancreas of E. sinensis with or without nitrite stress. Results showed that EsGST1-15 were all regulated by EsNrf2 with or without nitrite stress. Our study provides new information about the diversity, expression, and regulation of GSTs in E. sinensis under nitrite stress.
中华绒螯蟹是中国最重要的经济水产养殖品种之一。然而，亚硝酸盐污染已成为中华绒螯蟹健康养殖的严重威胁。谷胱甘肽 S-转移酶（GST）是一种重要的 II 相解毒酶，在细胞对外源物质的解毒中起主导作用。本研究从中华绒螯蟹中获得了 15 个 GST 基因（命名为 EsGST1-15），并研究了它们在亚硝酸盐胁迫下在中华绒螯蟹中的表达和调控。EsGST1-15 属于不同的 GST 亚类。EsGST1、EsGST2、EsGST3、EsGST4 和 EsGST5 属于 Delta 类 GST；EsGST6 和 EsGST7 为 Theta 类 GST；EsGST8 为 mGST-3 类 GST；EsGST9 属于 mGST-1 类 GST；EsGST10 和 EsGST11 属于 Sigma 类 GST；EsGST12、EsGST13 和 EsGST14 属于 Mu 类 GST；EsGST15 属于 Kappa 类 GST。组织分布实验表明，EsGSTs 在所有检测组织中广泛分布。在亚硝酸盐胁迫下，中华绒螯蟹肝胰腺中 EsGST1-15 的表达水平显著升高，表明 EsGSTs 参与了中华绒螯蟹在亚硝酸盐胁迫下的解毒过程。核因子-红细胞 2 相关因子 2（Nrf2）是一种转录因子，可激活解毒酶的表达。我们检测了在有或没有亚硝酸盐胁迫的情况下，用或不用 EsNrf2 干扰中华绒螯蟹肝胰腺后 EsGST1-15 的表达。结果表明，EsGST1-15 均受 EsNrf2 的调控，无论有无亚硝酸盐胁迫。本研究为亚硝酸盐胁迫下中华绒螯蟹 GSTs 的多样性、表达和调控提供了新的信息。
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