Yuan Yi, Wu Qianqian, Wang Xingran, Liu Mengyang, Yan Jiaqing, Ji Hui
School of Electrical Engineering, Yanshan University, Qinhuangdao 066004, China; Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province, Yanshan University, Qinhuangdao 066004, China.
School of Electrical Engineering, Yanshan University, Qinhuangdao 066004, China; Key Laboratory of Intelligent Rehabilitation and Neuromodulation of Hebei Province, Yanshan University, Qinhuangdao 066004, China.
Neuroimage. 2023 Apr 15;270:119979. doi: 10.1016/j.neuroimage.2023.119979. Epub 2023 Mar 1.
Previous studies have demonstrated that transcranial ultrasound stimulation (TUS) not only modulates cerebral hemodynamics, neural activity, and neurovascular coupling characteristics in resting samples but also exerts a significant inhibitory effect on the neural activity in task samples. However, the effect of TUS on cerebral blood oxygenation and neurovascular coupling in task samples remains to be elucidated. To answer this question, we first used forepaw electrical stimulation of the mice to elicit the corresponding cortical excitation, and then stimulated this cortical region using different modes of TUS, and simultaneously recorded the local field potential using electrophysiological acquisition and hemodynamics using optical intrinsic signal imaging. The results indicate that for the mice under peripheral sensory stimulation state, TUS with a duty cycle of 50% can (1) enhance the amplitude of cerebral blood oxygenation signal, (2) reduce the time-frequency characteristics of evoked potential, (3) reduce the strength of neurovascular coupling in time domain, (4) enhance the strength of neurovascular coupling in frequency domain, and (5) reduce the time-frequency cross-coupling of neurovasculature. The results of this study indicate that TUS can modulate the cerebral blood oxygenation and neurovascular coupling in peripheral sensory stimulation state mice under specific parameters. This study opens up a new area of investigation for potential applicability of TUS in brain diseases related to cerebral blood oxygenation and neurovascular coupling.
先前的研究表明，经颅超声刺激（TUS）不仅能调节静息样本中的脑血流动力学、神经活动和神经血管耦合特性，还能对任务样本中的神经活动产生显著的抑制作用。然而，TUS对任务样本中脑血氧合和神经血管耦合的影响仍有待阐明。为了回答这个问题，我们首先对小鼠进行前爪电刺激以引发相应的皮层兴奋，然后使用不同模式的TUS刺激该皮层区域，并同时使用电生理采集记录局部场电位，使用光学固有信号成像记录血流动力学。结果表明，对于处于外周感觉刺激状态的小鼠，占空比为50%的TUS能够：（1）增强脑血氧合信号的幅度；（2）降低诱发电位的时频特征；（3）降低时域神经血管耦合的强度；（4）增强频域神经血管耦合的强度；（5）降低神经血管的时频交叉耦合。本研究结果表明，TUS在特定参数下能够调节外周感觉刺激状态小鼠的脑血氧合和神经血管耦合。本研究为TUS在与脑血氧合和神经血管耦合相关的脑部疾病中的潜在应用开辟了一个新的研究领域。
