Zhu Lijun, Xiao Mingmei, Luo Jigang, Li Shijie, Liu Wenting, Wu Jinchuan, Song Zhuoyue
South China Research Center for Acupuncture and Moxibustion, Guangzhou University of Chinese Medicine, Guangzhou 510006, Guangdong, PR China.
Bioengineering Laboratory, Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510006, Guangdong, PR China.
Int J Biol Macromol. 2023 Apr 30;235:123853. doi: 10.1016/j.ijbiomac.2023.123853. Epub 2023 Feb 28.
The purpose of this study was to investigate the preventive effects of polysaccharide from Ostrea rivularis (ORP) on high-fat diet (HFD)-induced nonalcoholic fatty liver disease (NAFLD) in mice and the underlying mechanism. The results showed that NAFLD model group mice had significant fatty liver lesions. ORP could significantly reduce TC, TG and LDL level, and increase HDL level in serum of HFD mice. Besides, it could also reduce the contents of serum AST and ALT and alleviate pathological changes of fatty liver disease. ORP could also enhance the intestinal barrier function. 16sRNA analysis showed that ORP could reduce the abundance of Firmicutes and Proteobacteria and the ratio of Firmicutes/ Bacteroidetes at the phylum level. These results suggested that ORP could regulate the composition of gut microbiota in NAFLD mice, enhance intestinal barrier function, reduce intestinal permeability, and finally delay the progress and reduce the occurrence of NAFLD. In brief, ORP is an ideal polysaccharide for prevention and treatment of NAFLD, which can be developed as functional food or candidate drugs.
本研究旨在探讨近江牡蛎多糖（ORP）对高脂饮食（HFD）诱导的小鼠非酒精性脂肪性肝病（NAFLD）的预防作用及其潜在机制。结果显示，NAFLD模型组小鼠出现明显的肝脏脂肪病变。ORP可显著降低HFD小鼠血清中的总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白（LDL）水平，并提高高密度脂蛋白（HDL）水平。此外，它还可降低血清谷草转氨酶（AST）和谷丙转氨酶（ALT）的含量，减轻脂肪性肝病的病理变化。ORP还可增强肠道屏障功能。16sRNA分析表明，ORP可在门水平上降低厚壁菌门和变形菌门的丰度以及厚壁菌门/拟杆菌门的比例。这些结果提示，ORP可调节NAFLD小鼠肠道微生物群的组成，增强肠道屏障功能，降低肠道通透性，最终延缓NAFLD的进展并减少其发生。简而言之，ORP是预防和治疗NAFLD的理想多糖，可开发为功能性食品或候选药物。
