Canatar İpek, Zenger Okan, Özdaş Sibel, Baydemir Peşint Gözde
Faculty of Engineering Sciences, Department of Bioengineering, Adana Alpaslan Türkeş Science and Technology University, Adana, Turkey.
J Biomed Mater Res B Appl Biomater. 2023 Jun;111(6):1259-1270. doi: 10.1002/jbm.b.35230. Epub 2023 Mar 2.
Cryogels are support materials which are good at mimicking extracellular matrix due to their excellent hydrophilicity, biocompatibility, and macroporous structure, thus they are useful in facilitating cell activities during healing process. In this study, polyvinyl alcohol-gelatin (PVA-Gel) based cryogel membranes loaded with pterostilbene (trans-3,5-dimethoxy-4-hydroxystilbene; PTS) (PVA-Gel/PTS) was synthesized as wound dressing materials. PVA-Gel and PVA-Gel/PTS were synthesized with the polymerization yields of 96% ± 0.23% and 98% ± 0.18%, respectively, and characterized by swelling tests, Brunauer-Emmett-Teller (BET) and scanning electron microscopy (SEM) analysis. The swelling ratios were calculated as 98.6% ± 4.93% and 102% ± 5.1%, macroporosities were determined as 85% ± 2.13% and 88% ± 2.2%, for PVA-Gel and PVA-Gel/PTS, respectively. It was determined that PVA-Gel and PVA-Gel/PTS have 17 m /g ± 0.76 m /g and 20 m /g ± 0.92 m /g surface areas, respectively. SEM studies were demonstrated that they have ~100 μm pore sizes. According to 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), trypan blue exclusion and live-dead assay results, it was observed that cell proliferation, cell number and cell viability were higher in PVA-Gel/PTS cryogel at 24, 48, and 72 h compared to PVA-Gel. A strong and transparent fluorescent light intensity was observed indicating higher cell population in PVA-Gel/PTS in comparison with PVA-Gel, according to 4',6-diamidino-2-phenylindole (DAPI) staining. SEM, F-Actin, Giemsa staining and inverted-phase microscope image of fibroblasts in PVA-Gel/PTS cryogels revealed that dense fibroblast proliferation and spindle-shaped morphology of cells were preserved. Moreover, DNA agarose gel data demonstrated that PVA-Gel/PTS cryogels had no effect on DNA integrity. Consequently, produced PVA-Gel/PTS cryogel can be used as wound dressing material to promote wound therapies, inducing cell viability and proliferation.
冷冻凝胶是一种支撑材料，因其优异的亲水性、生物相容性和大孔结构而善于模拟细胞外基质，因此在促进愈合过程中的细胞活动方面很有用。在本研究中，合成了负载紫檀芪（反式 - 3,5 - 二甲氧基 - 4 - 羟基芪；PTS）的基于聚乙烯醇 - 明胶（PVA - Gel）的冷冻凝胶膜（PVA - Gel/PTS）作为伤口敷料材料。PVA - Gel和PVA - Gel/PTS的聚合产率分别为96% ± 0.23%和98% ± 0.18%，并通过溶胀试验、布鲁诺尔 - 埃米特 - 泰勒（BET）和扫描电子显微镜（SEM）分析进行表征。PVA - Gel和PVA - Gel/PTS的溶胀率分别计算为98.6% ± 4.93%和102% ± 5.1%，大孔率分别确定为85% ± 2.13%和88% ± 2.2%。确定PVA - Gel和PVA - Gel/PTS的表面积分别为17 m²/g ± 0.76 m²/g和20 m²/g ± 0.92 m²/g。SEM研究表明它们的孔径约为100μm。根据3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐（MTT）、台盼蓝排斥和活死细胞检测结果，观察到在24、48和72小时时，PVA - Gel/PTS冷冻凝胶中的细胞增殖、细胞数量和细胞活力均高于PVA - Gel。根据4',6 - 二脒基 - 2 - 苯基吲哚（DAPI）染色，观察到强烈且透明的荧光强度，表明与PVA - Gel相比，PVA - Gel/PTS中的细胞数量更多。PVA - Gel/PTS冷冻凝胶中成纤维细胞的SEM、F - 肌动蛋白、吉姆萨染色和倒置相差显微镜图像显示，细胞密集增殖且保持纺锤形形态。此外，DNA琼脂糖凝胶数据表明PVA - Gel/PTS冷冻凝胶对DNA完整性没有影响。因此，制备的PVA - Gel/PTS冷冻凝胶可作为伤口敷料材料用于促进伤口治疗，诱导细胞活力和增殖。
Zotero 插件