Department of Cellular Biochemistry, University Medical Center Göttingen, 37073 Göttingen, Germany.
Department of Cellular Biochemistry, University Medical Center Göttingen, 37073 Göttingen, Germany; Cluster of Excellence "Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells" (MBExC), 37073 University of Göttingen, Germany; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Translational Neuroinflammation and Automated Microscopy TNM, 37075 Göttingen, Germany; Max Planck Institute for Multidisciplinary Science, 37077 Göttingen, Germany.
Trends Cell Biol. 2023 Sep;33(9):765-772. doi: 10.1016/j.tcb.2023.02.001. Epub 2023 Feb 28.
Most mitochondrial proteins are synthesized in the cytosol and transported into mitochondria by protein translocases. Yet, mitochondria contain their own genome and gene expression system, which generates proteins that are inserted in the inner membrane by the oxidase assembly (OXA) insertase. OXA contributes to targeting proteins from both genetic origins. Recent data provides insights into how OXA cooperates with the mitochondrial ribosome during synthesis of mitochondrial-encoded proteins. A picture of OXA emerges in which it coordinates insertion of OXPHOS core subunits and their assembly into protein complexes but also participates in the biogenesis of select imported proteins. These functions position the OXA as a multifunctional protein insertase that facilitates protein transport, assembly, and stability at the inner membrane.
大多数线粒体蛋白是在细胞质中合成的，并通过蛋白转位酶转运到线粒体中。然而，线粒体含有自己的基因组和基因表达系统，该系统产生的蛋白质由氧化酶组装（OXA）插入酶插入内膜。OXA 有助于靶向来自两种遗传来源的蛋白质。最近的数据提供了关于 OXA 如何在合成线粒体编码蛋白期间与线粒体核糖体合作的见解。OXA 的作用方式是协调 OXPHOS 核心亚基的插入及其组装成蛋白复合物，但也参与某些输入蛋白的生物发生。这些功能使 OXA 成为一种多功能蛋白插入酶，可促进内膜处的蛋白转运、组装和稳定性。
