Applied Entomology and Zoology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, 21545, Egypt.
Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, 21545, Egypt.
Sci Rep. 2023 Mar 2;13(1):3542. doi: 10.1038/s41598-023-30588-x.
Finding innovative eco-friendly agents for pest control may be aided by investigating the plant-derived extracts' properties on economic pests. Therefore, the insecticidal, behavioral, biological and biochemical effects of Magnolia grandiflora (Magnoliaceae) leaf water and methanol extracts, Schinus terebinthifolius (Anacardiaceae) wood methanol extract, and Salix babylonica (Salicaceae) leaf methanol extract in comparison with a reference insecticide novaluron against S. littoralis were evaluated. The extracts were analyzed by High-Performance Liquid Chromatography (HPLC). The most abundant phenolic compounds were 4-hydroxybenzoic acid (7.16 mg/mL) and ferulic acid (6.34 mg/mL) in M. grandiflora leaf water extract; catechol (13.05 mg/mL), ferulic acid (11.87 mg/mL), and chlorogenic acid (10.33 mg/mL) in M. grandiflora leaf methanol extract; ferulic acid (14.81 mg/mL), caffeic acid (5.61 mg/mL), and gallic acid (5.07 mg/mL) In the S. terebinthifolius extract; cinnamic acid (11.36 mg/mL), and protocatechuic acid (10.33 mg/mL) In the methanol extract from S. babylonica extract. S. terebinthifolius extract had a highly toxic effect against second larvae after 96 h and eggs with LC values of 0.89 and 0.94 mg/L, respectively. Despite M. grandiflora extracts didn't show any toxicity against S. littoralis stages, they had an attractant effect on fourth- and second larvae, with feeding deterrence values of - 2.7% and - 6.7%, respectively, at 10 mg/L. S. terebinthifolius extract significantly reduced the percentage of pupation, adult emergence, hatchability, and fecundity, with values of 60.2%, 56.7%, 35.3%, and 105.4 eggs/female, respectively. Novaluron and S. terebinthifolius extract drastically inhibited the activities of α-amylase and total proteases to 1.16 and 0.52, and 1.47 and 0.65 ΔOD/mg protein/min, respectively. In the semi-field experiment, the residual toxicity of tested extracts on S. littoralis gradually decreased over time compared to novaluron. These findings indicate that extract from S. terebinthifolius is a promising insecticidal agent against S. littoralis.
为了寻找具有创新性的环保型害虫防治药剂，可以研究植物提取物对经济害虫的特性。因此，对玉兰（Magnoliaceae）叶水和甲醇提取物、巴西橡胶树（Anacardiaceae）木甲醇提取物和垂柳（Salicaceae）叶甲醇提取物的杀虫、行为、生物和生化效应进行了评估，并与参考杀虫剂novaluron 对沙蚕（S. littoralis）进行了比较。提取物通过高效液相色谱法（HPLC）进行分析。在玉兰叶水提取物中，最丰富的酚类化合物为 4-羟基苯甲酸（7.16mg/mL）和阿魏酸（6.34mg/mL）；在玉兰叶甲醇提取物中，最丰富的酚类化合物为儿茶酚（13.05mg/mL）、阿魏酸（11.87mg/mL）和绿原酸（10.33mg/mL）；在巴西橡胶树提取物中，最丰富的酚类化合物为阿魏酸（14.81mg/mL）、咖啡酸（5.61mg/mL）和没食子酸（5.07mg/mL）；在垂柳甲醇提取物中，最丰富的酚类化合物为肉桂酸（11.36mg/mL）和原儿茶酸（10.33mg/mL）。巴西橡胶树提取物对 96 小时后的第二代幼虫和卵具有极高的毒性，LC 值分别为 0.89 和 0.94mg/L。尽管玉兰提取物对沙蚕各阶段均无毒性，但它们对第四龄幼虫和第二龄幼虫具有引诱作用，在 10mg/L 时的摄食抑制率分别为-2.7%和-6.7%。巴西橡胶树提取物显著降低了化蛹率、成虫羽化率、孵化率和产卵量，分别为 60.2%、56.7%、35.3%和 105.4 个/雌虫。novaluron 和巴西橡胶树提取物将 α-淀粉酶和总蛋白酶的活性分别抑制到 1.16 和 0.52，以及 1.47 和 0.65 ΔOD/mg 蛋白/min。在半田间试验中，与 novaluron 相比，测试提取物对沙蚕的残留毒性随时间逐渐降低。这些发现表明，巴西橡胶树提取物是一种很有前途的沙蚕杀虫剂。
