Fibrinolysis in ascitic fluid: isolation and characterization of fibrin derivatives, their interaction with albumin.

A method for the preparative isolation of high molecular weight fibrin degradation products (XDP) from ascitic fluid of patients with advanced ovarian cancer is described. By non-reduced and reduced PAA-Gel electrophoresis we could demonstrate that high molecular weight fibrin derivatives exist as E-complexes. A similarity of the polypeptide chain composition from in vitro samples and ascitic fluid could be shown. Immunoadsorption with anti-albumin followed by Westernblotting with anti-fibrinogen-demonstrated the existence of XDP/albumin-associates in ascitic fluid.