Wilhelm O, Hafter R, Henschen A, Schmitt M, Graeff H
Frauenklinik der Technischen Universität München, FRG.
Blood. 1990 Apr 15;75(8):1673-8.
The aim of this study was to evaluate the type of enzymes involved in tumor-associated fibrinolysis of the stroma component fibrin in ovarian cancer patients. For this purpose, the high-molecular-mass fibrin degradation products (HMM-XDP) were isolated from malignant ascitic fluid by protamine sulfate precipitation and further purified by gel filtration and acid precipitation. After reduction with 2-mercaptoethanol, the peptide chain components were separated by reverse-phase high-performance liquid chromatography (RP-HPLC). The nature of these components was elucidated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and N-terminal amino acid sequence analysis and compared with fibrin-derived fragments formed in vitro. The results indicate that plasmin is the essential protease involved in the degradation of the stroma-derived fibrin portion found in ovarian cancer ascites.
本研究的目的是评估参与卵巢癌患者基质成分纤维蛋白肿瘤相关纤维蛋白溶解的酶的类型。为此，通过硫酸鱼精蛋白沉淀从恶性腹水中分离出高分子量纤维蛋白降解产物（HMM-XDP），并通过凝胶过滤和酸沉淀进一步纯化。用2-巯基乙醇还原后，通过反相高效液相色谱（RP-HPLC）分离肽链成分。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和N端氨基酸序列分析阐明这些成分的性质，并与体外形成的纤维蛋白衍生片段进行比较。结果表明，纤溶酶是参与卵巢癌腹水中基质衍生纤维蛋白部分降解的关键蛋白酶。
