Gezer Arzu, Karadağ Sari Ebru
Vocational School of Health Services, Atatürk University, Erzurum, Turkey.
Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey.
Iran J Basic Med Sci. 2023 Mar;26(3):276-284. doi: 10.22038/IJBMS.2023.65948.14507.
The present study aims to determine how various dosages of chronic roflumilast affect testicular tissue and testosterone levels in healthy rats.
Biochemical tests, along with histopathological, immunohistochemical, and immunofluorescence studies, were carried out.
Loss of tissue in the seminiferous epithelium, degeneration in the interstitial area, a separation between cells, desquamation, interstitial edema, and degenerative alterations in testicular tissue were observed in roflumilast groups when compared with the other groups. While apoptosis and autophagy were statistically negligible in the control and sham groups, the roflumilast groups had significantly higher apoptotic and autophagic alterations, as well as immunopositivity. Serum testosterone levels in the 1 mg/kg roflumilast group were lower than those in the control, sham, and 0.5 mg/kg roflumilast groups.
Analyses of the research findings revealed that continuous usage of the broad-spectrum active component roflumilast exerted unfavorable effects on the testicular tissue and testosterone levels of rats.
本研究旨在确定不同剂量的慢性罗氟司特对健康大鼠睾丸组织和睾酮水平的影响。
进行了生化测试以及组织病理学、免疫组织化学和免疫荧光研究。
与其他组相比，罗氟司特组观察到生精上皮组织丢失、间质区变性、细胞分离、脱屑、间质水肿以及睾丸组织的退行性改变。虽然对照组和假手术组的凋亡和自噬在统计学上可忽略不计，但罗氟司特组的凋亡和自噬改变以及免疫阳性率显著更高。罗氟司特1mg/kg组的血清睾酮水平低于对照组、假手术组和罗氟司特0.5mg/kg组。
对研究结果的分析表明，持续使用广谱活性成分罗氟司特对大鼠的睾丸组织和睾酮水平产生了不利影响。
