Belhan Saadet, Özkaraca Mustafa, Özdek Uğur, Kömüroğlu Ahmet Ufuk
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Van Yuzuncu Yıl University, Van, Turkey.
Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey.
Andrologia. 2020 Oct;52(9):e13747. doi: 10.1111/and.13747. Epub 2020 Jul 16.
This study investigated the role of chrysin (CR) in DNA damage likely to occur in the testicle and oxidative stress caused by doxorubicin (DXR). Twenty-eight rats were divided into four groups as control, DXR, DXR + CR and CR groups. Sperm parameters, oxidative status, testicular biopsy score, DNA damage and plasma testosterone levels were analysed. Noticeable reductions in sperm count, motility and testosterone were detected in the DXR group compared to controls. In addition, significant increases in malondialdehyde (MDA), catalase (CAT) and glutathione (GSH) levels, and in abnormal sperm rates were detected. Severe degenerative changes occurred in the tubules of DXR rat testes; the inter-tubular areas were oedematous. Immunofluorescence staining was conducted with 8-OhDG (8 oxo-2'-deoxyguanosine) to evaluate DNA damage, and severe positivity was found in tubular gaps in the DXR rat testes. When the DXR + CR group was compared with the DXR group, the abnormal sperm rate was found to have decreased significantly. Positivity in the tubular space and degenerative changes in the seminiferous tubules were also diminished. We recommend the administration of CR with DXR to reduce the possible adverse effects of DXR, a medicine preferred in cancer therapy.
本研究调查了白杨素（CR）在睾丸可能发生的DNA损伤以及阿霉素（DXR）引起的氧化应激中的作用。28只大鼠分为四组：对照组、DXR组、DXR + CR组和CR组。分析精子参数、氧化状态、睾丸活检评分、DNA损伤和血浆睾酮水平。与对照组相比，DXR组精子数量、活力和睾酮水平明显降低。此外，丙二醛（MDA）、过氧化氢酶（CAT）和谷胱甘肽（GSH）水平以及异常精子率显著升高。DXR组大鼠睾丸小管出现严重退行性改变；小管间区域水肿。用8-OhDG（8-氧代-2'-脱氧鸟苷）进行免疫荧光染色以评估DNA损伤，发现DXR组大鼠睾丸小管间隙呈强阳性。将DXR + CR组与DXR组比较，发现异常精子率显著降低。小管间隙的阳性反应和生精小管的退行性改变也有所减轻。我们建议在使用DXR（一种癌症治疗中常用的药物）时加用CR，以减少DXR可能产生的不良反应。
