Leach C L, Hatoum N S, Ratajczak H V, Gerhart J M
IIT Research Institute, Life Sciences Research, Chicago, IL 60616.
Toxicol Lett. 1987 Dec;39(2-3):189-98. doi: 10.1016/0378-4274(87)90232-3.
Four groups of 40 male Sprague-Dawley rats each were exposed by inhalation to target concentrations of 0, 0.1, 1.0, and 3.0 ppm of acrolein 6 h/day, 5 days/week for 3 weeks. Subsequent changes in local pulmonary immunity were determined by examining the number of antibody plaque-forming cells in the lung-associated lymph nodes following intratracheal immunization with sheep red blood cells. Separate groups of rats were evaluated for blastogenic responsiveness to phytohemagglutinin-P and Salmonella typhimurium antigen using spleen- and lung-associated lymph node cells. In vivo resistance was evaluated utilizing acrolein-exposed rats subsequently challenged with intravenous Listeria monocytogenes. Local pulmonary antibody responsiveness was not affected by acrolein exposure. Lymphocyte blastogenesis and resistance to Listeria challenge were not altered. Body weights and spleen weights were decreased in the 3 ppm-exposed group only. Microscopic examination of the nasal turbinates revealed acrolein-induced exfoliation, erosion, and necrosis of the respiratory epithelium as well as squamous metaplasia, however, lung histology was not affected. Thus at environmental concentrations, acrolein toxicity appeared to be confined to local nasal pathologic changes with no alterations in lung histology or immune function.
将四组每组40只雄性斯普拉格-道利大鼠，每天6小时、每周5天、持续3周通过吸入暴露于浓度分别为0、0.1、1.0和3.0 ppm的丙烯醛环境中。在用绵羊红细胞进行气管内免疫后，通过检查肺相关淋巴结中抗体形成细胞的数量来确定局部肺免疫的后续变化。使用脾细胞和肺相关淋巴结细胞，对单独的几组大鼠进行对植物血凝素-P和鼠伤寒沙门氏菌抗原的致有丝分裂反应性评估。利用经丙烯醛暴露后再用静脉注射单核细胞增生李斯特菌攻击的大鼠评估体内抵抗力。丙烯醛暴露未影响局部肺抗体反应性。淋巴细胞增殖和对李斯特菌攻击的抵抗力未改变。仅在暴露于3 ppm组中体重和脾脏重量有所下降。对鼻甲的显微镜检查显示，丙烯醛可导致呼吸上皮的剥脱、糜烂和坏死以及鳞状化生，然而，肺组织学未受影响。因此，在环境浓度下，丙烯醛毒性似乎仅限于局部鼻腔病理变化，而肺组织学和免疫功能未改变。
