Department of Endodontics, Gulhane Faculty of Dentistry, University of Health Sciences, Etlik, Keçiören, 06018, Ankara, Turkey.
Department of Stomatology, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Máximo S/N., 18071, Granada, Spain.
Clin Oral Investig. 2023 Jun;27(6):3181-3188. doi: 10.1007/s00784-023-04929-z. Epub 2023 Mar 3.
The impact of conservative instrumentation on the disinfection of root canals with different curvatures has not yet been determined. This ex vivo study aimed to evaluate and compare the effect of conservative instrumentation with TruNatomy (TN) and Rotate and a conventional rotary system, ProTaper Gold (PTG), on root canal disinfection during chemomechanical preparation of straight and curved canals.
Ninety mandibular molars with straight (n = 45) and curved (n = 45) mesiobuccal root canals were contaminated with polymicrobial clinical samples. Teeth were divided into three subgroups (n = 14) according to the file systems and the curvature. Canals were instrumented with TN, Rotate, and PTG, respectively. Sodium hypochlorite and EDTA were used as irrigants. Intracanal samples were taken before (S1) and after (S2) instrumentation. Six uninfected teeth were used as negative controls. The bacterial reduction between S1 and S2 was measured by ATP assay, flow cytometry, and culture methods. Kruskal-Wallis and ANOVA tests were followed by the Duncan post hoc test (p < 0.05).
Bacterial reduction percentages were similar for the three file systems in straight canals (p > 0.05). However, PTG showed a lower reduction percentage of intact membrane cells in flow cytometry than TN and Rotate (p = 0.036). For the curved canals, no significant differences were obtained (p > 0.05).
Conservative instrumentation of straight and curved canals using TN and Rotate files resulted in similar bacterial reduction compared to PTG.
The disinfection efficacy of conservative instrumentation is similar to conventional instrumentation in straight and curved root canals.
目前尚未确定保形锉对不同弯曲度根管消毒的影响。本离体研究旨在评估和比较 TruNatomy（TN）和Rotate 与传统的 ProTaper Gold（PTG）旋转锉系统在直弯根管化学机械预备时对根管消毒的效果。
90 颗下颌磨牙的近中颊根管为直形（n=45）和弯曲形（n=45），均受到多微生物临床样本的污染。根据锉系统和弯曲度将牙齿分为三组（n=14）。分别用 TN、Rotate 和 PTG 进行根管预备。次氯酸钠和 EDTA 用作冲洗液。分别在预备前（S1）和预备后（S2）采集根管内样本。6 颗未感染的牙齿用作阴性对照。通过 ATP 测定、流式细胞术和培养方法测量 S1 和 S2 之间的细菌减少量。采用 Kruskal-Wallis 和 ANOVA 检验，然后进行 Duncan 事后检验（p<0.05）。
在直形根管中，三种锉系统的细菌减少率相似（p>0.05）。然而，与 TN 和 Rotate 相比，PTG 在流式细胞术中显示出对完整膜细胞的较低减少百分比（p=0.036）。对于弯曲根管，未获得显著差异（p>0.05）。
与 PTG 相比，使用 TN 和 Rotate 锉对直形和弯曲根管进行保形锉预备可获得相似的细菌减少效果。
保形锉在直弯根管中的消毒效果与传统锉相似。
