Deng Ya, Yang Guangfeng, Lens Piet N L, He Yaxue, Qie Lingxiang, Shen Xingyu, Chen Jianmeng, Cheng Zhuowei, Chen Dongzhi
School of Petrochemical Engineering and Environment, Zhejiang Ocean University, Zhoushan 316004, China; College of Environment, Zhejiang University of Technology, Hangzhou 310032, China.
School of Petrochemical Engineering and Environment, Zhejiang Ocean University, Zhoushan 316004, China.
J Hazard Mater. 2023 May 15;450:131063. doi: 10.1016/j.jhazmat.2023.131063. Epub 2023 Feb 21.
Mass transfer limitation usually causes the poor performance of biotrickling filters (BTFs) for the treatment of hydrophobic volatile organic compounds (VOCs) during long-term operation. In this study, two identical lab-scale BTFs were established to remove a mixture of n-hexane and dichloromethane (DCM) gases using non-ionic surfactant Tween 20 by Pseudomonas mendocina NX-1 and Methylobacterium rhodesianum H13. A low pressure drop (≤110 Pa) and a rapid biomass accumulation (17.1 mg g) were observed in the presence of Tween 20 during the startup period (30 d). The removal efficiency (RE) of n-hexane was enhanced by 15.0%- 20.5% while DCM was completely removed with the inlet concentration (IC) of 300 mg·m at different empty bed residence times in the Tween 20 added BTF. The viable cells and the relative hydrophobicity of the biofilm were increased under the action of Tween 20, which facilitated the mass transfer and enhanced the metabolic utilization of pollutants by microbes. Besides, Tween 20 addition enhanced the biofilm formation processes including the increased extracellular polymeric substance (EPS) secretion, biofilm roughness and biofilm adhesion. The kinetic model simulated the removal performance of the BTF with Tween 20 for the mixed hydrophobic VOCs, and the goodness-of-fit was above 0.9.
传质限制通常会导致生物滴滤器（BTF）在长期运行过程中处理疏水性挥发性有机化合物（VOC）的性能不佳。在本研究中，建立了两个相同的实验室规模的BTF，以利用门多萨假单胞菌NX-1和罗得西亚甲基杆菌H13通过非离子表面活性剂吐温20去除正己烷和二氯甲烷（DCM）气体的混合物。在启动期（30天），在吐温20存在的情况下，观察到低压降（≤110 Pa）和快速的生物量积累（17.1 mg g）。在添加吐温20的BTF中，在不同的空床停留时间下，正己烷的去除效率（RE）提高了15.0%-20.5%，而当入口浓度（IC）为300 mg·m时，DCM被完全去除。在吐温20的作用下，生物膜的活细胞和相对疏水性增加，这促进了传质并增强了微生物对污染物的代谢利用。此外，添加吐温20增强了生物膜形成过程，包括细胞外聚合物（EPS）分泌增加、生物膜粗糙度和生物膜附着力。动力学模型模拟了添加吐温20的BTF对混合疏水性VOCs的去除性能，拟合优度高于0.9。
