Wang Jiannan, Lin Yaling, Yang Ju, Zhang Qiang, Liu Maili, Hu Yunfei, Dong Xu
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan National Laboratory for Optoelectronics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Biochem Biophys Res Commun. 2023 Apr 23;653:133-139. doi: 10.1016/j.bbrc.2023.02.072. Epub 2023 Feb 26.
The Arabidopsis WRKY11 (AtWRKY11) protein is an important transcription factor involved in plant response to biotic and abiotic stresses. Its DNA-binding domain specifically binds to gene promoter regions harboring the W-box consensus motif. Herein we report the high-resolution structure of the AtWRKY11 DNA-binding domain (DBD) determined by solution NMR spectroscopy. The results show that AtWRKY11-DBD adopts an all-β fold comprising five β-strands packed in an antiparallel topology, stabilized by a zinc-finger motif. Structural comparison reveals that the long β-β loop shows the highest structural variation from other available WRKY domain structures. Moreover, this loop was further found to contribute to the binding between AtWRKY11-DBD and W-box DNA. Our current study provides atomic-level structural basis for further understanding the structure-function relationship of plant WRKY proteins.
拟南芥WRKY11（AtWRKY11）蛋白是一种重要的转录因子，参与植物对生物和非生物胁迫的响应。其DNA结合结构域特异性结合含有W-box共有基序的基因启动子区域。在此，我们报道了通过溶液核磁共振光谱法测定的AtWRKY11 DNA结合结构域（DBD）的高分辨率结构。结果表明，AtWRKY11-DBD采用由五个β链以反平行拓扑结构堆积而成的全β折叠结构，由锌指基序稳定。结构比较表明，长的β-β环与其他可用的WRKY结构域结构相比具有最高的结构变化。此外，进一步发现该环有助于AtWRKY11-DBD与W-box DNA之间的结合。我们目前的研究为进一步理解植物WRKY蛋白的结构-功能关系提供了原子水平的结构基础。
