Ataturk University, Faculty of Veterinary Medicine, Department of Parasitology, Erzurum, Turkey..
Ataturk University, Faculty of Veterinary Medicine, Department of Parasitology, Erzurum, Turkey.
Res Vet Sci. 2023 Apr;157:40-49. doi: 10.1016/j.rvsc.2023.02.012. Epub 2023 Feb 28.
Piroplasmosis is a tick-borne protozoan disease caused by Babesia and Theileria species in ruminants. This study sought to determine the presence and prevalence of the agents causing piroplasmosis among sheep in Erzurum province, Turkey. It also sought to identify the tick species infesting the sheep and investigate the possible role of the ticks in the transmission of piroplasmosis. A total of 1621 blood samples and 1696 ixodid ticks from infested sheep were collected. Each blood sample and 115 tick pools were subjected to PCR assay. A total of 307 blood samples were found to be positive for Babesia spp. and Theileria spp. according to molecular analysis. The sequence analysis was revealed the presence of B. ovis (0.4%), B. crassa (0.4%), B. canis (0.4%), T. ovis (69.3%), Theileria sp. (26.6%), and Theileria sp. OT3 (2.9%) in 244 samples. The collected ticks were identified as D. marginatus (62.5%), Hae. parva (36.2%), Hae. punctata (1.1%), Rh. turanicus (0.1%), and H. marginatum (0.1%). The molecular analysis of the adult tick samples revealed T. ovis and T. annulata positivity in the D. marginatus pools, B. crassa and T. ovis positivity in the Hae. parva pools, and T. ovis positivity in the Hae. punctata pools. These results provide up-to-date data concerning tick-borne protozoan diseases of sheep and tick species infesting sheep in the region. The sheep breeding industry is an important livelihood for the region so it is essential to perform repeated studies on these pathogens in order to prevent disruptions to animal husbandry.
梨形虫病是一种由巴贝斯虫和泰勒虫引起的蜱传原生动物病，发生于反刍动物。本研究旨在确定土耳其埃尔祖鲁姆省绵羊中引起梨形虫病的病原体的存在和流行情况。本研究还旨在确定感染绵羊的蜱种，并调查蜱在传播梨形虫病中的可能作用。共采集了 1621 份血液样本和 1696 只感染的绵羊伊蚊。每个血液样本和 115 个蜱群都进行了 PCR 检测。根据分子分析，共有 307 份血液样本对巴贝斯虫属和泰勒虫属呈阳性。序列分析显示，在 244 个样本中存在 B.ovis(0.4%)、B.crassa(0.4%)、B.canis(0.4%)、T.ovis(69.3%)、Theileria sp.(26.6%)和 Theileria sp.OT3(2.9%)。采集的蜱种鉴定为 D.marginatus(62.5%)、Hae.parva(36.2%)、Hae.punctata(1.1%)、Rh.turanicus(0.1%)和 H.marginatum(0.1%)。对成年蜱样本的分子分析显示，D.marginatus 蜱群中存在 T.ovis 和 T.annulata 阳性，Hae.parva 蜱群中存在 B.crassa 和 T.ovis 阳性，Hae.punctata 蜱群中存在 T.ovis 阳性。这些结果提供了有关该地区绵羊蜱传原生动物病和感染绵羊的蜱种的最新数据。绵羊养殖是该地区的重要生计，因此，必须对这些病原体进行反复研究，以防止畜牧业受到干扰。
