Department of Pharmacognosy, Faculty of Pharmacy, University of Lagos, Lagos, Nigeria.
Central Research Laboratory, University of Lagos, Lagos, Nigeria.
J Ethnopharmacol. 2023 Jun 12;309:116337. doi: 10.1016/j.jep.2023.116337. Epub 2023 Mar 1.
Daniellia oliveri (Rolfe) Hutch. & Dalziel (Fabaceae) is used for the treatment of inflammatory diseases and pains (chest pain, toothache and lumbago) and rheumatism.
The study investigates the anti-inflammatory and antinociceptive properties of D. oliveri and possible mechanism of antiinflammatory action.
Acute toxicity of the extract was evaluated in mice using the limit test. The anti-inflammatory activity was assessed in xylene-induced paw oedema and carrageenan-induced air-pouch models at doses of 50, 100 and 200 mg/kg, p.o. Volume of exudate, total protein, leukocyte counts, myeloperoxidase (MPO) and concentration of cytokines (TNF-α and IL-6) were measured in the exudate of rats in the carrageenan-induced air-pouch model. Other parameters include lipid peroxidation (LPO), nitric oxide (NO) and antioxidant indices (SOD, CAT and GSH). Histopathology of the air pouch tissue was also carried out. The antinociceptive effect was assessed using acetic acid-induced writhing, tail flick and formalin tests. Locomotor activity was done in the open field test. The extract was analysed with HPLC-DAD-UV technique.
The extract showed significant anti-inflammatory effect (73.68 and 75.79%, inhibition) in xylene-induced ear oedema test at the dose of 100 and 200 mg/kg, respectively. In carrageenan air pouch model, the extract significantly reduced exudate volume, protein concentration, the migration of leukocytes and MPO production in the exudate. The concentrations of cytokines TNF-α (12.25 ± 1.80 pg/mL) and IL-6 (21.12 pg/mL) in the exudate at the dose of 200 mg/kg were reduced compared to carrageenan alone group (48.15 ± 4.50 pg/mL; 82.62 pg/mL) respectively. The extract showed significant increase in the activities of CAT and SOD and GSH concentration. The histopathological assessment of the pouch lining revealed reduction of immuno-inflammatory cell influx. Nociception was significantly inhibited by the extract in acetic acid-induced writhing model and the second phase of formalin test indicating a peripheral mechanism of action. The open field test showed that D. oliveri did not alter locomotor activity. The acute toxicity study did not cause mortality or signs of toxicity at 2000 mg/kg, p.o. We identified and quantified caffeic acid, p-coumaric acid, ferulic acid, rutin, apigenin-7-glucoside, quercetin and kaempferol in the extract.
The results of our study showed that the stem bark extract of D. oliveri possesses anti-inflammatory and antinociceptive activities thereby supporting its traditional use in the treatment of some inflammatory and painful disorders.
丹尼利奥利弗（Rolfe）哈钦。和 Dalziel（豆科）用于治疗炎症性疾病和疼痛（胸痛，牙痛和腰痛）和风湿病。
本研究调查了丹尼利奥利弗的抗炎和镇痛特性以及抗炎作用的可能机制。
使用极限试验在小鼠中评估提取物的急性毒性。在 50、100 和 200mg/kg、po 剂量下，通过二甲苯诱导的爪水肿和角叉菜胶诱导的气囊模型评估抗炎活性。在角叉菜胶诱导的气囊模型中，测量大鼠渗出液中的体积、总蛋白、白细胞计数、髓过氧化物酶（MPO）和细胞因子（TNF-α和 IL-6）的浓度。其他参数包括脂质过氧化（LPO）、一氧化氮（NO）和抗氧化指数（SOD、CAT 和 GSH）。还进行了气囊组织的组织病理学检查。使用乙酸诱导的扭体、尾巴拍打和福马林试验评估镇痛作用。在旷场试验中进行了运动活动。使用 HPLC-DAD-UV 技术分析提取物。
提取物在二甲苯诱导的耳肿胀试验中在 100 和 200mg/kg 剂量下分别显示出显著的抗炎作用（73.68%和 75.79%，抑制）。在角叉菜胶气囊模型中，提取物显著减少渗出液体积、蛋白浓度、白细胞迁移和渗出液中 MPO 的产生。与角叉菜胶单独组（48.15±4.50pg/mL；82.62pg/mL）相比，在 200mg/kg 剂量下，渗出液中细胞因子 TNF-α（12.25±1.80pg/mL）和 IL-6（21.12pg/mL）的浓度降低。提取物显示 CAT 和 SOD 的活性以及 GSH 浓度显著增加。气囊衬里的组织病理学评估显示免疫炎症细胞浸润减少。提取物在乙酸诱导的扭体模型和福马林试验的第二阶段显著抑制伤害感受，表明其具有外周作用机制。旷场试验表明，D. oliveri 不会改变运动活动。在 2000mg/kg、po 时，急性毒性研究未导致死亡或毒性迹象。我们在提取物中鉴定和定量了咖啡酸、对香豆酸、阿魏酸、芦丁、芹菜素-7-葡萄糖苷、槲皮素和山奈酚。
我们的研究结果表明，丹尼利奥利弗的茎皮提取物具有抗炎和镇痛活性，因此支持其在治疗一些炎症和疼痛性疾病中的传统用途。
