Jin Qing-Ri, Mao Jing-Wei, Zhu Fei
School of Pharmacy, Hangzhou Medical College, Hangzhou, 311399, China.
Key Laboratory of Applied Technology of Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A＆F University, Hangzhou, 311300, China.
Fish Shellfish Immunol. 2023 Apr;135:108655. doi: 10.1016/j.fsi.2023.108655. Epub 2023 Mar 1.
In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain. Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type. These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays. Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes. Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes. HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs. HFPs maintained peroxidase activity after WSSV challenge, thereby providing protection against oxidative damage caused by the virus. HFPs also promoted apoptosis of hemocytes after WSSV infection. In addition, HFPs significantly enhanced the survival rate of WSSV-infected crabs. All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis. Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
在本研究中，我们从羊栖菜中提取了多糖（HFPs），并评估了它们对拟穴青蟹免疫反应的影响。成分分析表明，HFPs作为硫酸化多糖，主要由甘露糖醛酸（49.05%）和岩藻糖（22.29%）组成，糖链结构为β型。这些结果表明，HFPs在体内或体外试验中具有潜在的抗氧化和免疫刺激活性。通过本研究，我们发现HFPs抑制了感染白斑综合征病毒（WSSV）的螃蟹体内病毒的复制，并促进了血细胞对溶藻弧菌的吞噬作用。定量PCR结果表明，HFPs上调了螃蟹血细胞中astakine、crustin、肌球蛋白、MCM7、STAT、TLR、JAK、CAP和p53的表达水平。HFPs还促进了超氧化物歧化酶和酸性磷酸酶的活性以及螃蟹血淋巴的抗氧化活性。在WSSV攻击后，HFPs维持了过氧化物酶的活性，从而提供了对病毒引起的氧化损伤的保护。HFPs还促进了WSSV感染后血细胞的凋亡。此外，HFPs显著提高了感染WSSV的螃蟹的存活率。所有结果证实，HFPs通过增强抗菌肽的表达、抗氧化酶活性、吞噬作用和凋亡来提高拟穴青蟹的先天免疫力。因此，HFPs有潜力用作治疗或预防剂，以调节拟穴青蟹的先天免疫力并保护它们免受微生物感染。
