Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agriculture and Forestry University, Hangzhou, China.
Front Immunol. 2022 Jan 27;12:812717. doi: 10.3389/fimmu.2021.812717. eCollection 2021.
MicroRNAs (miRNAs) are important signaling regulators that are involved in regulating the innate immunity of crustacean. However, few studies focus on the role of crustacean miRNAs in the cellular immunity have been reported. In this study, we showed that the expression of miR-133 was significantly up-regulated in the mud crab after infection by white spot syndrome virus (WSSV) or . The anti-miRNA oligonucleotide AMO-miR-133 was used to knock down miR-133 expression in . The number of WSSV copies increased significantly in WSSV-infected crabs after miR-133 knockdown. Knockdown of miR-133 also enhanced the mortality rates of WSSV-infected and infected mud crabs, and it significantly enhanced the expression of the astakine, which was confirmed by real-time quantitative PCR and western blot analysis. The data also indicate that miR-133 may affect hemocyte proliferation in by regulating ne expression. miR-133 Knockdown enhanced the apoptosis or phagocytosis of crab hemocytes, and increased the mortality of mud crabs after WSSV or infection. These results indicate that miR-133 is involved in the host immune response to WSSV and infection in mud crabs. Taken together, our research provides new insights for the control of viral or vibrio diseases in
微小 RNA（miRNAs）是重要的信号调节因子，参与调控甲壳动物的固有免疫。然而，目前关于甲壳动物 miRNAs 在细胞免疫中的作用的研究较少。本研究显示，在白斑综合征病毒（WSSV）或感染后，泥蟹中的 miR-133 表达显著上调。反义 miRNA 寡核苷酸 AMO-miR-133 用于敲低 miR-133 的表达。miR-133 敲低后，WSSV 感染蟹体内的 WSSV 拷贝数显著增加。miR-133 敲低还增强了 WSSV 感染和感染泥蟹的死亡率，并通过实时定量 PCR 和 Western blot 分析证实了它显著增强了 astakine 的表达。数据还表明，miR-133 可能通过调节 ne 表达来影响血细胞的增殖。miR-133 敲低增强了蟹血细胞的凋亡或吞噬作用，并增加了 WSSV 或感染后泥蟹的死亡率。这些结果表明，miR-133 参与了泥蟹对 WSSV 和感染的宿主免疫反应。总之，我们的研究为控制病毒性或弧菌病提供了新的思路。
