Jiang Bo, Wu Chunyan, Liang Yuan, Li Xiaofeng, Li Jie, Song Guibo
Yunnan Key Laboratory of Laboratory Medicine, Kunming, 650032, China.
Yunnan Province Clinical Research Center for Laboratory Medicine, Kunming, 650032, China.
Curr Microbiol. 2023 Mar 4;80(4):123. doi: 10.1007/s00284-023-03218-y.
Pseudomonas aeruginosa is a widespread source of hospital-acquired infections and a top priority antibiotic-resistant pathogen as it has developed robust immunity to most traditional antibiotics. Quorum sensing (QS) enables P. aeruginosa to modulate virulence functions and is important for pathogenesis. QS relies on the production and perception of autoinducing chemical signal molecules. Acyl-homoserine lactones are the key autoinducer molecules that mediate P. aeruginosa-associated QS, and N-(3-oxododecanoyl)-L-homoserine lactone (3-O-C12-HSL) and N-butyryl-L-homoserine lactone (C4-HSL) are the two types. This study aimed to identify potential quenching targets of QS pathways that may reduce the chances of resistance developing in P. aeruginosa using co-culture approaches. In co-cultures, Bacillus reduced the production of 3-O-C12-HSL/C4-HSL signal molecules by inactivating acyl- homoserine lactone-based QS to inhibit important virulence factor expression. Moreover, Bacillus is subject to complex crosstalk with other regulatory systems, such as the integrated QS system and the Iqs system. The results showed that blocking one or more QS pathways was insufficient to reduce infection with multidrug resistant P. aeruginosa.
铜绿假单胞菌是医院获得性感染的广泛来源，也是首要的抗生素耐药病原体，因为它已对大多数传统抗生素产生了强大的耐药性。群体感应（QS）使铜绿假单胞菌能够调节毒力功能，对发病机制很重要。群体感应依赖于自诱导化学信号分子的产生和感知。酰基高丝氨酸内酯是介导铜绿假单胞菌相关群体感应的关键自诱导分子，N-（3-氧代十二烷酰基）-L-高丝氨酸内酯（3-O-C12-HSL）和N-丁酰-L-高丝氨酸内酯（C4-HSL）是两种类型。本研究旨在通过共培养方法确定群体感应途径的潜在淬灭靶点，这些靶点可能会降低铜绿假单胞菌产生耐药性的几率。在共培养中，芽孢杆菌通过使基于酰基高丝氨酸内酯的群体感应失活来抑制重要毒力因子的表达，从而减少3-O-C12-HSL/C4-HSL信号分子 的产生。此外，芽孢杆菌会与其他调节系统发生复杂的相互作用，如整合群体感应系统和Iqs系统。结果表明，阻断一条或多条群体感应途径不足以减少多重耐药铜绿假单胞菌的感染。
