Sang Wei, Huang Songfeng, Chen Jiajie, Dai Xiaoqi, Liu Haoyu, Zeng Youjun, Zhang Teliang, Wang Xueliang, Qu Junle, Ho Ho-Pui, Shao Yonghong
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, 518060, China.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronics Engineering, Shenzhen University, Shenzhen, 518060, China.
Talanta. 2023 Jun 1;258:124405. doi: 10.1016/j.talanta.2023.124405. Epub 2023 Mar 1.
Phase interrogation surface plasmon resonance (P-SPR) biosensors have the highest sensitivity among different types of surface plasmon resonance (SPR) biosensors. However, P-SPR sensors have small dynamic detection range and complex device configuration. To solve these two problems, we designed a multi-channel P-SPR imaging (mcP-SPRi) sensing platform based on a common-path ellipsometry scheme. A wavelength sequential selection (WSS) technique for P-SPRi sensing is developed to select the optimal sensing wavelengths according to different refractive indexes (RIs) of the samples, so the inconsistency of SPR signal response for different biomolecule types caused by the small dynamic detection range is eliminated. And a dynamic detection range of 3.7×10 RIU is achieved, which is the largest among the current mcP-SPRi biosensors. Remarkably, the individual SPR phase image acquisition time has been greatly reduced to 1s by using WSS method instead of whole spectrum scanning, which enables the high-throughput mcP-SPRi sensing.
相位询问表面等离子体共振（P-SPR）生物传感器在不同类型的表面等离子体共振（SPR）生物传感器中具有最高的灵敏度。然而，P-SPR传感器的动态检测范围小且设备配置复杂。为了解决这两个问题，我们基于共光路椭偏测量方案设计了一种多通道P-SPR成像（mcP-SPRi）传感平台。开发了一种用于P-SPRi传感的波长顺序选择（WSS）技术，以根据样品的不同折射率（RI）选择最佳传感波长，从而消除了由小动态检测范围导致的不同生物分子类型的SPR信号响应不一致的问题。并且实现了3.7×10 RIU的动态检测范围，这在当前的mcP-SPRi生物传感器中是最大的。值得注意的是，通过使用WSS方法而非全光谱扫描，单个SPR相位图像采集时间已大幅缩短至1秒，这使得高通量mcP-SPRi传感成为可能。
