Deng Shijie, Wang Peng, Yu Xinglong
State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, Beijing 100084, China.
Sensors (Basel). 2017 Dec 5;17(12):2819. doi: 10.3390/s17122819.
Surface plasmon resonance (SPR) is an optical sensing technique that is capable of performing real-time, label-free and high-sensitivity monitoring of molecular interactions. SPR biosensors can be divided according to their operating principles into angle-, wavelength-, intensity- and phase-interrogated devices. With their complex optical configurations, phase-interrogated SPR sensors generally provide higher sensitivity and throughput, and have thus recently emerged as prominent biosensing devices. To date, several methods have been developed for SPR phase interrogation, including heterodyne detection, polarimetry, shear interferometry, spatial phase modulation interferometry and temporal phase modulation interferometry. This paper summarizes the fundamentals of phase-sensitive SPR sensing, reviews the available methods for phase interrogation of these sensors, and discusses the future prospects for and trends in the development of this technology.
表面等离子体共振（SPR）是一种光学传感技术，能够对分子相互作用进行实时、无标记和高灵敏度监测。SPR生物传感器可根据其工作原理分为角度询问型、波长询问型、强度询问型和相位询问型设备。由于其复杂的光学配置，相位询问型SPR传感器通常具有更高的灵敏度和通量，因此最近已成为突出的生物传感设备。迄今为止，已开发出几种用于SPR相位询问的方法，包括外差检测、偏振测量、剪切干涉测量、空间相位调制干涉测量和时间相位调制干涉测量。本文总结了相敏SPR传感的基本原理，回顾了这些传感器相位询问的可用方法，并讨论了该技术发展的未来前景和趋势。
