Gao Xiang, Du Jing, Cheng Li, Li Zhaofeng, Li Caiming, Ban Xiaofeng, Gu Zhengbiao, Hong Yan
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Wuxi 214122, Jiangsu Province, People's Republic of China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, People's Republic of China; Collaborative Innovation Center for Food Safety and Quality Control, Jiangnan University, Wuxi 214122, Jiangsu Province, People's Republic of China.
Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Wuxi 214122, Jiangsu Province, People's Republic of China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, People's Republic of China; Collaborative Innovation Center for Food Safety and Quality Control, Jiangnan University, Wuxi 214122, Jiangsu Province, People's Republic of China.
Int J Biol Macromol. 2023 May 1;236:123907. doi: 10.1016/j.ijbiomac.2023.123907. Epub 2023 Mar 3.
In this study, octenyl succinic anhydride (OSA) starch with different folic acid (FA) grafting time was prepared and the degree of FA substitution at different grafting time was determined. The results of XPS quantitatively reflected the surface elemental composition of OSA starch grafted with FA. FTIR spectra further confirmed the successful introduction of FA on OSA starch granules. SEM images showed that the surface roughness of OSA starch granules was more obvious with higher FA grafting time. The particle size, zeta potential, and swelling properties were determined to study the effect of FA on the structure of OSA starch. TGA indicated that FA effectively enhanced the thermal stability of OSA starch at high temperature. The crystalline form of the OSA starch gradually transformed from A type to a hybrid A and V-type with the progress of FA grafting reaction. In addition, the anti-digestive properties of OSA starch were enhanced after grafting FA. Using doxorubicin hydrochloride (DOX) as the model drug, the loading efficiency of OSA starch grafted with FA for DOX reached 87.71 %. These results provide novel insights into OSA starch grafted with FA as potential strategy for loading DOX.
在本研究中，制备了具有不同叶酸（FA）接枝时间的辛烯基琥珀酸酐（OSA）淀粉，并测定了不同接枝时间下的FA取代度。X射线光电子能谱（XPS）结果定量反映了接枝FA的OSA淀粉的表面元素组成。傅里叶变换红外光谱（FTIR）进一步证实了FA成功引入到OSA淀粉颗粒上。扫描电子显微镜（SEM）图像显示，随着FA接枝时间的增加，OSA淀粉颗粒的表面粗糙度更加明显。测定了粒径、zeta电位和溶胀性能，以研究FA对OSA淀粉结构的影响。热重分析（TGA）表明，FA在高温下有效提高了OSA淀粉的热稳定性。随着FA接枝反应的进行，OSA淀粉晶型逐渐从A型转变为A和V型混合晶型。此外，接枝FA后OSA淀粉的抗消化性能增强。以盐酸多柔比星（DOX）为模型药物，接枝FA的OSA淀粉对DOX的载药效率达到87.71%。这些结果为接枝FA的OSA淀粉作为载药DOX的潜在策略提供了新的见解。
