• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

沙眼衣原体质粒编码的 Pgp3 蛋白诱导 HeLa 宫颈上皮细胞分泌不同的炎症特征。

Chlamydia trachomatis plasmid-encoding Pgp3 protein induces secretion of distinct inflammatory signatures from HeLa cervical epithelial cells.

机构信息

Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.

Department of Obstetrics and Gynecology, Faculty of Medicine, Universiti Malaya, 50603, Kuala Lumpur, Malaysia.

出版信息

BMC Microbiol. 2023 Mar 4;23(1):58. doi: 10.1186/s12866-023-02802-3.

DOI:10.1186/s12866-023-02802-3
PMID:36870960
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9985209/
Abstract

BACKGROUND

Genital Chlamydia trachomatis infection is the most common bacterial sexual transmitted disease that causes severe complications including pelvic inflammatory disease, ectopic pregnancy, and infertility in females. The Pgp3 protein encoded by C. trachomatis plasmid has been speculated to be an important player in chlamydial pathogenesis. However, the precise function of this protein is unknown and thus remains to be thoroughly investigated.

METHODS

In this study, we synthesized Pgp3 protein for in vitro stimulation in the Hela cervical carcinoma cells.

RESULTS AND CONCLUSION

We showed that Pgp3 induced prominent expression of host inflammatory cytokine genes including interleukin-6 (IL-6), IL-8, tumor necrosis factor alpha-induced protein 3 (TNFAIP3), and chemokine C-X-C motif ligand 1 (CXCL1), implying a possible role of Pgp3 in modulating the inflammatory reaction in the host.

摘要

背景

生殖道沙眼衣原体感染是最常见的细菌性性传播疾病，可导致严重并发症，包括女性盆腔炎、宫外孕和不孕。推测沙眼衣原体质粒编码的 Pgp3 蛋白在衣原体发病机制中起重要作用。然而，该蛋白的确切功能尚不清楚，因此仍需深入研究。

方法

本研究中，我们合成了 Pgp3 蛋白用于体外刺激宫颈癌细胞系 Hela。

结果与结论

结果表明，Pgp3 诱导宿主炎症细胞因子基因（包括白细胞介素 6、白细胞介素 8、肿瘤坏死因子-α诱导蛋白 3 和趋化因子 C-X-C 基元配体 1）的显著表达，提示 Pgp3 可能在调节宿主炎症反应中发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37d3/9985209/ef5b5052e7b3/12866_2023_2802_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37d3/9985209/7381c69218e2/12866_2023_2802_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37d3/9985209/d46a9ab01327/12866_2023_2802_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37d3/9985209/ef5b5052e7b3/12866_2023_2802_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37d3/9985209/7381c69218e2/12866_2023_2802_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37d3/9985209/d46a9ab01327/12866_2023_2802_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37d3/9985209/ef5b5052e7b3/12866_2023_2802_Fig3_HTML.jpg

相似文献

1
Chlamydia trachomatis plasmid-encoding Pgp3 protein induces secretion of distinct inflammatory signatures from HeLa cervical epithelial cells.沙眼衣原体质粒编码的 Pgp3 蛋白诱导 HeLa 宫颈上皮细胞分泌不同的炎症特征。
BMC Microbiol. 2023 Mar 4;23(1):58. doi: 10.1186/s12866-023-02802-3.
2
Chlamydia trachomatis Plasmid Gene Protein 3 Is Essential for the Establishment of Persistent Infection and Associated Immunopathology.沙眼衣原体质粒基因蛋白 3 对于建立持续性感染和相关免疫病理学是必需的。
mBio. 2020 Aug 18;11(4):e01902-20. doi: 10.1128/mBio.01902-20.
3
Antiapoptotic activity of Chlamydia trachomatis Pgp3 protein involves activation of the ERK1/2 pathway mediated by upregulation of DJ-1 protein.沙眼衣原体 Pgp3 蛋白的抗细胞凋亡活性涉及 DJ-1 蛋白上调介导的 ERK1/2 通路的激活。
Pathog Dis. 2019 Dec 1;77(9). doi: 10.1093/femspd/ftaa003.
4
Antibodies from women urogenitally infected with C. trachomatis predominantly recognized the plasmid protein pgp3 in a conformation-dependent manner.泌尿生殖道感染沙眼衣原体的女性体内的抗体主要以构象依赖的方式识别质粒蛋白pgp3。
BMC Microbiol. 2008 Jun 9;8:90. doi: 10.1186/1471-2180-8-90.
5
Chlamydial plasmid-encoded virulence factor Pgp3 interacts with human cathelicidin peptide LL-37 to modulate immune response.衣原体质粒编码的毒力因子 Pgp3 与人源抗菌肽 LL-37 相互作用，调节免疫反应。
Microbes Infect. 2019 Jan-Feb;21(1):50-55. doi: 10.1016/j.micinf.2018.06.003. Epub 2018 Jun 26.
6
Chitosan particle stabilized Pickering emulsion/interleukin-12 adjuvant system for Pgp3 subunit vaccine elicits immune protection against genital chlamydial infection in mice.壳聚糖颗粒稳定的 Pickering 乳液/白细胞介素-12 佐剂系统用于 Pgp3 亚单位疫苗，可诱导小鼠对生殖道衣原体感染产生免疫保护。
Front Immunol. 2022 Nov 23;13:989620. doi: 10.3389/fimmu.2022.989620. eCollection 2022.
7
Activation of neutrophils by Chlamydia trachomatis-infected epithelial cells is modulated by the chlamydial plasmid.沙眼衣原体感染的上皮细胞激活中性粒细胞的过程受衣原体质粒的调节。
Microbes Infect. 2018 May;20(5):284-292. doi: 10.1016/j.micinf.2018.02.007. Epub 2018 Mar 2.
8
Structure of the Chlamydia trachomatis immunodominant antigen Pgp3.沙眼衣原体免疫显性抗原 Pgp3 的结构。
J Biol Chem. 2013 Jul 26;288(30):22068-79. doi: 10.1074/jbc.M113.475012. Epub 2013 May 23.
9
Virulence Protein Pgp3 Is Insufficient To Mediate Plasmid-Dependent Infectivity of Chlamydia trachomatis.毒力蛋白 Pgp3 不足以介导沙眼衣原体依赖质粒的感染力。
Infect Immun. 2023 Feb 16;91(2):e0039222. doi: 10.1128/iai.00392-22. Epub 2023 Feb 1.
10
Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to Invasive Biovars.质粒对 CPAF 表达的负调控与 Pgp4 无关，且仅限于侵袭性生物型。
mBio. 2018 Jan 30;9(1):e02164-17. doi: 10.1128/mBio.02164-17.

引用本文的文献

1
Evidence for the presence of Borrelia burgdorferi in invasive breast cancer tissues.侵袭性乳腺癌组织中存在伯氏疏螺旋体的证据。
Eur J Microbiol Immunol (Bp). 2024 Mar 7;14(2):143-153. doi: 10.1556/1886.2024.00021. Print 2024 May 14.
2
Insights into innate immune cell evasion by .对 逃避免疫细胞先天免疫的深入洞察。
Front Immunol. 2024 Jan 25;15:1289644. doi: 10.3389/fimmu.2024.1289644. eCollection 2024.
3
Tryptophan residue of plasmid-encoded Pgp3 is important for to induce hydrosalpinx in mice.质粒编码的Pgp3的色氨酸残基对于在小鼠中诱导输卵管积水很重要。

本文引用的文献

1
The Nature and Extent of Plasmid Variation in .……中质粒变异的性质和程度
Microorganisms. 2020 Mar 6;8(3):373. doi: 10.3390/microorganisms8030373.
2
Combining individual Chlamydia trachomatis IgG antibodies MOMP, TARP, CPAF, OMP2, and HSP60 for tubal factor infertility prediction.联合检测沙眼衣原体 IgG 抗体 MOMP、TARP、CPAF、OMP2 和 HSP60 对输卵管性不孕的预测价值。
Am J Reprod Immunol. 2019 Mar;81(3):e13091. doi: 10.1111/aji.13091. Epub 2019 Feb 6.
3
CPAF, HSP60 and MOMP antigens elicit pro-inflammatory cytokines production in the peripheral blood mononuclear cells from genital Chlamydia trachomatis-infected patients.
Front Microbiol. 2023 Jul 11;14:1216372. doi: 10.3389/fmicb.2023.1216372. eCollection 2023.
4
Infection Remodels Host Cell Mitochondria to Alter Energy Metabolism and Subvert Apoptosis.感染重塑宿主细胞线粒体以改变能量代谢并颠覆细胞凋亡。
Microorganisms. 2023 May 24;11(6):1382. doi: 10.3390/microorganisms11061382.
CPAF、HSP60 和 MOMP 抗原可引起生殖道沙眼衣原体感染患者外周血单个核细胞产生促炎细胞因子。
Immunobiology. 2019 Jan;224(1):34-41. doi: 10.1016/j.imbio.2018.10.010. Epub 2018 Nov 20.
4
Chlamydial plasmid-encoded virulence factor Pgp3 interacts with human cathelicidin peptide LL-37 to modulate immune response.衣原体质粒编码的毒力因子 Pgp3 与人源抗菌肽 LL-37 相互作用，调节免疫反应。
Microbes Infect. 2019 Jan-Feb;21(1):50-55. doi: 10.1016/j.micinf.2018.06.003. Epub 2018 Jun 26.
5
The Relationship between Chlamydia trachomatis Genital Infection and Spontaneous Abortion.沙眼衣原体生殖道感染与自然流产之间的关系
J Reprod Infertil. 2016 Apr-Jun;17(2):110-6.
6
Molecular characterisation of the Chlamydia pecorum plasmid from porcine, ovine, bovine, and koala strains indicates plasmid-strain co-evolution.来自猪、羊、牛和考拉菌株的嗜肺衣原体质粒的分子特征表明质粒与菌株共同进化。
PeerJ. 2016 Feb 4;4:e1661. doi: 10.7717/peerj.1661. eCollection 2016.
7
Global Estimates of the Prevalence and Incidence of Four Curable Sexually Transmitted Infections in 2012 Based on Systematic Review and Global Reporting.基于系统评价和全球报告的2012年四种可治愈性传播感染的患病率和发病率全球估计
PLoS One. 2015 Dec 8;10(12):e0143304. doi: 10.1371/journal.pone.0143304. eCollection 2015.
8
TLR2/MyD88/NF-κB signalling pathway regulates IL-8 production in porcine alveolar macrophages infected with porcine circovirus 2.Toll样受体2/髓样分化因子88/核因子κB信号通路调控猪圆环病毒2感染的猪肺泡巨噬细胞中白细胞介素-8的产生。
J Gen Virol. 2016 Feb;97(2):445-452. doi: 10.1099/jgv.0.000345. Epub 2015 Nov 17.
9
Genetic diversity in the plasticity zone and the presence of the chlamydial plasmid differentiates Chlamydia pecorum strains from pigs, sheep, cattle, and koalas.可塑性区域的遗传多样性以及衣原体质粒的存在区分了来自猪、绵羊、牛和考拉的鹦鹉热衣原体菌株。
BMC Genomics. 2015 Nov 4;16:893. doi: 10.1186/s12864-015-2053-8.
10
Chlamydial plasmid-encoded virulence factor Pgp3 neutralizes the antichlamydial activity of human cathelicidin LL-37.衣原体质粒编码的毒力因子Pgp3可中和人杀菌肽LL-37的抗衣原体活性。
Infect Immun. 2015 Dec;83(12):4701-9. doi: 10.1128/IAI.00746-15. Epub 2015 Sep 28.