Lomlim Luelak, Manuschai Jutharat, Ratti Pichayaporn, Kara Jiraporn, Sakunphueak Athip, Panichayupakaranant Pharkphoom, Naorungroj Supawadee
Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Phytomedicine and Pharmaceutical Biotechnology Excellent Center (PPBEC), Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Heliyon. 2023 Feb 20;9(3):e13919. doi: 10.1016/j.heliyon.2023.e13919. eCollection 2023 Mar.
The purpose of this study was to (i) synthesize and develop an alkynyloxy derivative of lawsone as an antifungal spray and (ii) assess the antifungal spray's effectiveness in reducing the viability of () on polymethylmethacrylate (PMMA) specimens.
Lawsone methyl ether (LME) and its derivative, 2-(prop-2-ynyloxy)naphthalene-1,4-dione (compound ) were synthesized and characterized. The synthetic compounds were screened for antimicrobial activities against using the microtiter broth dilution method to determine the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Compound was further formulated as an antifungal spray in three concentrations (100, 200, and 400 μg/mL). biofilms were developed for 48 h on PMMA specimens. The efficacy of using an antifungal spray for 1 and 3 min to remove biofilm was assessed using colony counting and scanning electron microscopy (SEM). Chlorhexidine gluconate (CHX), polident®, and distilled water were used as positive and negative control cleansing solutions, respectively.
LME and compound showed comparable inhibition against with a MIC of 25 μg/mL and MFC of 50 μg/mL. For immediate treatment, was not detected on PMMA specimens when expose to 2% CHX and compound (100, 200, and 400 μg/mL) antifungal spray for 3 min. However, after recolonization, a small number of viable cells were observed in denture soaked in compound antifungal spray for 3 min group. Following recolonization, polident® and distilled water had comparable viable cell counts of to the no treatment group. Scanning electron microscope (SEM) images revealed that CHX, polident®, and compound caused cell damage in various forms.
Denture spray containing synthetic alkynyloxy derivative of lawsone is a promising antifungal agent for biofilm removal from the PMMA surface.
本研究的目的是：（i）合成并开发一种作为抗真菌喷雾剂的胡桃醌炔氧基衍生物，以及（ii）评估该抗真菌喷雾剂在降低聚甲基丙烯酸甲酯（PMMA）标本上的（某种真菌，原文未明确给出具体名称）生存能力方面的有效性。
合成并表征了胡桃醌甲醚（LME）及其衍生物2-(丙-2-炔氧基)萘-1,4-二酮（化合物 ，原文未明确给出具体编号）。使用微量肉汤稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MFC），对合成化合物针对（某种真菌，原文未明确给出具体名称）的抗菌活性进行筛选。化合物 进一步配制成三种浓度（100、200和400μg/mL）的抗真菌喷雾剂。在PMMA标本上培养48小时形成生物膜。使用菌落计数和扫描电子显微镜（SEM）评估使用抗真菌喷雾剂1分钟和3分钟去除生物膜的效果。分别使用葡萄糖酸氯己定（CHX）、Polident® 和蒸馏水作为阳性和阴性对照清洁溶液。
LME和化合物 对（某种真菌，原文未明确给出具体名称）表现出相当的抑制作用，MIC为25μg/mL，MFC为50μg/mL。对于即时治疗，当暴露于2% CHX和化合物 （100、200和400μg/mL）抗真菌喷雾剂3分钟时，在PMMA标本上未检测到（某种真菌，原文未明确给出具体名称）。然而，在再定植后，在浸泡于化合物 抗真菌喷雾剂3分钟的假牙组中观察到少量活细胞。再定植后，Polident® 和蒸馏水的活细胞数与未处理组相当。扫描电子显微镜（SEM）图像显示，CHX、Polident® 和化合物 导致了各种形式的细胞损伤。
含有合成胡桃醌炔氧基衍生物的假牙喷雾剂是一种有前景的抗真菌剂，可用于从PMMA表面去除（某种真菌，原文未明确给出具体名称）生物膜。
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