壳聚糖低分子量对聚甲基丙烯酸甲酯树脂上白色念珠菌生物膜的功效。

Efficacy of low-molecular weight chitosan against Candida albicans biofilm on polymethyl methacrylate resin.

机构信息

Department of Prosthodontics, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

Department of Microbiology and Research unit on Oral Microbiology and Immunology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.

出版信息

Aust Dent J. 2021 Sep;66(3):262-269. doi: 10.1111/adj.12826. Epub 2021 Feb 14.

DOI:10.1111/adj.12826

PMID:33486770

Abstract

BACKGROUND

Candida biofilm is a major cause of denture stomatitis. We aimed to compare the efficacy of low-molecular-weight chitosan solutions against Candida albicans biofilm on polymethyl methacrylate (PMMA) resin.

METHODS

Various types of chitosan were tested for anti-Candida activity by broth dilution. Two types were selected for further testing on 24-hour C.albicans biofilm formed on PMMA specimens. Specimens were randomly distributed among experimental groups, including 0.1% and 0.2% acetic acid, 3 and 6 mg/mL of oligomer chitosan and 30 kDa chitosan solutions, effervescent tablet (Polident), and 0.2% chlorhexidine, and immersed for 5 min to 12 h. The viability of C. albicans after cleansing were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. Remaining viability was calculated into percentage relative to respective controls and analyzed using ANOVA with Tukey post-hoc tests. Live/dead fluorescence microscopy was also performed.

RESULTS

Chitosan solutions had high efficacy against C. albicans biofilm on PMMA. The mean relative viability compared to control after 12-h immersion was 6.60 ± 4.75% and 12.72 ± 6.96% for 3 and 6 mg/mL oligomer, respectively, and 11.68 ± 4.81% and 18.08 ± 6.20% for 3 and 6 mg/mL 30 kDa chitosan, respectively.

CONCLUSIONS

Low-molecular-weight chitosan solution is an effective antifungal denture cleanser that can significantly reduce C. albicans viability in biofilm on PMMA.

摘要

背景

白色念珠菌生物膜是义齿性口炎的主要原因。我们旨在比较针对聚甲基丙烯酸甲酯（PMMA）树脂上白色念珠菌生物膜的低分子量壳聚糖溶液的疗效。

方法

通过肉汤稀释法测试各种类型的壳聚糖的抗念珠菌活性。选择两种类型进一步测试在 PMMA 标本上形成的 24 小时白色念珠菌生物膜。标本随机分布在实验组中，包括 0.1％和 0.2％的乙酸、3 和 6mg/mL 的低聚物壳聚糖和 30kDa 壳聚糖溶液、泡腾片（Polident）和 0.2％洗必泰，并浸泡 5 分钟至 12 小时。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）测定清洁后白色念珠菌的活力。将剩余活力计算为相对于各自对照的百分比，并使用 ANOVA 和 Tukey 事后检验进行分析。还进行了死活荧光显微镜检查。