Department of Oral Medicine, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing, China.
Department of Stomatology, Peking University Third Hospital, Beijing, China.
BMC Oral Health. 2020 Jul 8;20(1):193. doi: 10.1186/s12903-020-01176-6.
To compare the antifungal action of two commercially available denture cleaning agents to that of standard clinical solutions, and determine their effects on the polymethyl methacrylate (PMMA) acrylic resin denture surface.
Candida albicans growth was analyzed by colony forming assay, and the methyl thiazolyl tetrazolium (MTT) assay was used to evaluate biofilm formation and cell adhesion. The morphology and roughness of PMMA acrylic resin surface was measured by scanning electron microscopy (SEM) images and stylus method.
Clene®, Polident® and 3% NaHCO solutions showed significantly greater antifungal effects in terms of both inhibiting growth and biofilm formation. In addition, Clene® solution prevented adhesion of C. albicans on cell culture plates compared to filter-sterile tap water, whereas other reagents did not have an inhibitory effect. One-month immersion in the different cleaning reagents significantly inhibited fungal adhesion on the PMMA surface Clene®, Polident® and 3% NaHCO showed greater effect compared to PBS and filter-sterile tap water. Finally, none of the cleansing agents significantly affected the morphology and roughness of the PMMA surface.
Clene®, Polident® and 3% NaHCO solutions can inhibit C. albicans growth and biofilm formation to some extent on cell culture plates, and significantly inhibit fungal adhesion on the PMMA surface without affecting surface morphology and roughness.
比较两种市售义齿清洁剂与标准临床溶液的抗真菌作用，并确定它们对聚甲基丙烯酸甲酯（PMMA）树脂义齿表面的影响。
通过集落形成测定法分析白色念珠菌的生长情况，并通过甲基噻唑基四唑（MTT）测定法评估生物膜形成和细胞黏附。通过扫描电子显微镜（SEM）图像和触针法测量 PMMA 树脂表面的形貌和粗糙度。
Clene®、Polident®和 3%碳酸氢钠溶液在抑制生长和生物膜形成方面均表现出显著更强的抗真菌作用。此外，与过滤无菌自来水相比，Clene®溶液可防止白色念珠菌在细胞培养板上的黏附，而其他试剂则没有抑制作用。在不同清洁剂中浸泡一个月后，PMMA 表面的真菌黏附显著受到抑制。Clene®、Polident®和 3%碳酸氢钠的效果优于 PBS 和过滤无菌自来水。最后，没有一种清洁剂显著影响 PMMA 表面的形态和粗糙度。
Clene®、Polident®和 3%碳酸氢钠溶液在细胞培养板上可在一定程度上抑制白色念珠菌的生长和生物膜形成，并显著抑制 PMMA 表面的真菌黏附，而不影响表面形态和粗糙度。
