Ismaili Djemail, Schulz Carl, Horváth András, Koivumäki Jussi T, Mika Delphine, Hansen Arne, Eschenhagen Thomas, Christ Torsten
Institute of Experimental Pharmacology and Toxicology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Department of Cardiology, University Heart and Vascular Center Hamburg, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Front Physiol. 2023 Feb 16;14:1132165. doi: 10.3389/fphys.2023.1132165. eCollection 2023.
Models based on human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) are proposed in almost any field of physiology and pharmacology. The development of human induced pluripotent stem cell-derived cardiomyocytes is expected to become a step forward to increase the translational power of cardiovascular research. Importantly they should allow to study genetic effects on an electrophysiological background close to the human situation. However, biological and methodological issues revealed when human induced pluripotent stem cell-derived cardiomyocytes were used in experimental electrophysiology. We will discuss some of the challenges that should be considered when human induced pluripotent stem cell-derived cardiomyocytes will be used as a physiological model.
几乎在生理学和药理学的任何领域都有人提出基于人诱导多能干细胞衍生心肌细胞（hiPSC-CM）的模型。人诱导多能干细胞衍生心肌细胞的发展有望成为提高心血管研究转化能力的重要一步。重要的是，它们应有助于在接近人类实际情况的电生理背景下研究基因效应。然而，在实验电生理学中使用人诱导多能干细胞衍生心肌细胞时发现了生物学和方法学问题。我们将讨论在将人诱导多能干细胞衍生心肌细胞用作生理模型时应考虑的一些挑战。
