Xu Qiyun, Wu Zhongzhen, Zeng Xinnian, An Xincheng
Guangdong Engineering Research Center for Insect Behavior Regulation, College of Agriculture, South China Agricultural University, Guangzhou, China.
Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China.
Front Physiol. 2020 Jun 19;11:720. doi: 10.3389/fphys.2020.00720. eCollection 2020.
The black soldier fly, , is a cosmopolitan insect of the family Stratiomyidae (Diptera). Chemosensory genes encode proteins involved directly in the detection of odorants. In this study, we sequenced the antennal transcriptome of adults to identify chemosensory genes. Putative unigenes encoding 27 odorant binding proteins (OBPs), five chemosensory proteins (CSPs), 70 odorant receptors (ORs), 25 ionotropic receptors (IRs), 10 gustatory receptors (GRs) and two sensory neuron membrane proteins (SNMPs) were identified. Tissue-specific expression profiles of the identified OBPs, CSPs and SNMPs were investigated using RT-PCR. Eight OBPs (, , , and ), one CSP () and one SNMP () were predominantly expressed in antennae. Further real-time quantitative PCR analyses revealed that the antennae-enriched unigenes also exhibited significant differences in expression between males and females. Among the sex-biased unigenes, six ORs showed female-biased expression, suggesting that these genes might participate in female-specific behaviors such as oviposition site searching. Sixteen ORs and two OBPs showed male-biased expression, indicating that they may play key roles in the detection of female sex pheromones. Our study is the first attempt to delineate the molecular basis of chemoreception in . Our data provide useful information for comparative studies on the differentiation and evolution of Dipteran chemosensory gene families.
黑水虻是双翅目水虻科的一种世界性昆虫。化学感应基因编码直接参与气味剂检测的蛋白质。在本研究中，我们对黑水虻成虫的触角转录组进行了测序，以鉴定化学感应基因。鉴定出了推定的单基因，它们编码27种气味结合蛋白（OBP）、5种化学感应蛋白（CSP）、70种气味受体（OR）、25种离子型受体（IR）、10种味觉受体（GR）和2种感觉神经元膜蛋白（SNMP）。使用逆转录聚合酶链反应（RT-PCR）研究了所鉴定的OBP、CSP和SNMP的组织特异性表达谱。8种OBP（、、、和）、1种CSP（）和1种SNMP（）主要在触角中表达。进一步的实时定量PCR分析表明，在触角中富集的单基因在雄性和雌性之间的表达也存在显著差异。在有性别偏向的单基因中，6种OR表现出雌性偏向表达，这表明这些基因可能参与了诸如寻找产卵位点等雌性特异性行为。16种OR和2种OBP表现出雄性偏向表达，表明它们可能在检测雌性性信息素中起关键作用。我们的研究是首次尝试描绘黑水虻化学感受的分子基础。我们的数据为双翅目化学感应基因家族的分化和进化的比较研究提供了有用信息。
