Institute of Parasitology, Biomedical Research Center Seltersberg (BFS), Justus Liebig University Giessen, Giessen, Germany.
Institute of Anatomy and Cell Biology, Justus Liebig University Giessen, Giessen, Germany.
Front Immunol. 2023 Feb 16;14:1125667. doi: 10.3389/fimmu.2023.1125667. eCollection 2023.
Neutrophil extracellular traps (NET) formation is one important host innate defense mechanism elicited by polymorphonuclear neutrophils (PMN). NETs are composed by chromatin and proteins with microbicidal and signaling activity. So far, there is one report on triggered NETs in cattle, however, exact mechanisms, including signalling pathways and dynamics governing this reaction remain largely unknown. Recently, involvement of cell cycle proteins was demonstrated for phorbol myristate acetate (PMA)-triggered human PMN-derived NETs. Here, we studied the involvement of cell cycle proteins in -induced NETs in exposed bovine PMN. Through confocal and transmission electron microscopy we discovered that Ki-67 and lamin B1 signals are upregulated and relocated during -induced NETosis. Nuclear membrane disruption was also observed as a hallmark of NET formation in bovine PMN confronted with viable tachyzoites, mimicking some steps of mitosis. However, we did not observe centrosome duplication as previously described for human PMN-derived NET formation stimulated with PMA.
中性粒细胞胞外诱捕网（NET）的形成是多形核粒细胞（PMN）引发的宿主固有防御机制之一。NET 由具有杀菌和信号活性的染色质和蛋白质组成。到目前为止，已有一份关于牛中性粒细胞触发的 NET 的报告，然而，包括信号通路和控制这种反应的动力学在内的确切机制在很大程度上仍然未知。最近，已经证明细胞周期蛋白参与了佛波醇肉豆蔻酸酯（PMA）触发的人 PMN 衍生的 NET。在这里，我们研究了细胞周期蛋白在暴露的牛 PMN 中诱导的 NET 中的参与。通过共聚焦和透射电子显微镜，我们发现 Ki-67 和 lamin B1 信号在诱导的 NET 形成过程中被上调和重定位。核膜破裂也被观察到，这是牛 PMN 与活速殖子接触时形成 NET 的标志，模拟了有丝分裂的一些步骤。然而，我们没有观察到中心体复制，如先前用 PMA 刺激人 PMN 衍生的 NET 形成时所描述的那样。
