Yunaini Luluk, Ari Pujianto Dwi
Doctoral Program of Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta Pusat, Indonesia.
Department of Medicine Biology, Faculty of Medicine, Universitas Indonesia, Jakarta Pusat, Indonesia.
Int J Reprod Biomed. 2023 Feb 8;21(1):17-32. doi: 10.18502/ijrm.v21i1.12662. eCollection 2023 Jan.
The identification and characterization of relevant targets are necessary for developing nonhormonal male contraceptives. The molecules must demonstrate that they are necessary for reproduction. As a result, a sophisticated technique is required to identify the molecular targets for nonhormonal male contraceptives. Genetic modification (GM) techniques are one method that can be applied. This technique has been widely used to study gene function that effected male fertility and has resulted in the discovery of numerous nonhormonal male contraceptive target molecules. We examined GM techniques and approaches used to investigate genes involved in male fertility as potential targets for nonhormonal contraceptives. The discovery of nonhormonal contraceptive candidate molecules was increased by using GM techniques, especially the Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 method. The discovery of candidate nonhormonal contraceptive molecules can be a wide-open research for the development of nonhormonal male contraceptives. Therefore, we are believing that one day nonhormonal male contraceptives will be released.
确定和表征相关靶点对于开发非激素男性避孕药是必要的。这些分子必须证明它们对生殖是必需的。因此，需要一种复杂的技术来识别非激素男性避孕药的分子靶点。基因编辑（GM）技术是一种可以应用的方法。该技术已被广泛用于研究影响男性生育能力的基因功能，并导致发现了许多非激素男性避孕靶点分子。我们研究了用于研究参与男性生育的基因作为非激素避孕药潜在靶点的基因编辑技术和方法。通过使用基因编辑技术，特别是成簇规律间隔短回文重复序列/Cas9方法，增加了非激素避孕候选分子的发现。非激素避孕候选分子的发现对于非激素男性避孕药的开发可能是一个广阔的研究领域。因此，我们相信有一天非激素男性避孕药将会问世。
