Cheng Jin, Lin Lian, Yu Jiangtao, Zhu Xiaolu, Ma Haoli, Zhao Yan
Emergency Center, Zhongnan Hospital of Wuhan University, Wuhan, China.
Hubei Clinical Research Center for Emergency and Resuscitation, Zhongnan Hospital of Wuhan University, Wuhan, China.
Front Neurosci. 2023 Feb 15;17:1069640. doi: 10.3389/fnins.2023.1069640. eCollection 2023.
Recent studies have suggested a role for N6-methyladenosine (m6A) modification in neurological diseases. Hypothermia, a commonly used treatment for traumatic brain injury, plays a neuroprotective role by altering m6A modifications. In this study, methylated RNA immunoprecipitation sequencing (MeRIP-Seq) was applied to conduct a genome-wide analysis of RNA m6A methylation in the rat hippocampus of Sham and traumatic brain injury (TBI) groups. In addition, we identified the expression of mRNA in the rat hippocampus after TBI with hypothermia treatment. Compared with the Sham group, the sequencing results of the TBI group showed that 951 different m6A peaks and 1226 differentially expressed mRNAs were found. We performed cross-linking analysis of the data of the two groups. The result showed that 92 hyper-methylated genes were upregulated, 13 hyper-methylated genes were downregulated, 25 hypo-methylated genes were upregulated, and 10 hypo-methylated genes were downregulated. Moreover, a total of 758 differential peaks were identified between TBI and hypothermia treatment groups. Among these differential peaks, 173 peaks were altered by TBI and reversed by hypothermia treatment, including Plat, Pdcd5, Rnd3, Sirt1, Plaur, Runx1, Ccr1, Marveld1, Lmnb2, and Chd7. We found that hypothermia treatment transformed some aspects of the TBI-induced m6A methylation landscape of the rat hippocampus.
最近的研究表明N6-甲基腺苷（m6A）修饰在神经疾病中发挥作用。低温是治疗创伤性脑损伤常用的方法，通过改变m6A修饰发挥神经保护作用。在本研究中，应用甲基化RNA免疫沉淀测序（MeRIP-Seq）对假手术组和创伤性脑损伤（TBI）组大鼠海马体中的RNA m6A甲基化进行全基因组分析。此外，我们还鉴定了低温治疗后TBI大鼠海马体中mRNA的表达。与假手术组相比，TBI组的测序结果显示发现了951个不同的m6A峰和1226个差异表达的mRNA。我们对两组数据进行了交联分析。结果显示，92个高甲基化基因上调，13个高甲基化基因下调，25个低甲基化基因上调，10个低甲基化基因下调。此外，在TBI组和低温治疗组之间共鉴定出758个差异峰。在这些差异峰中，173个峰因TBI而改变，并因低温治疗而逆转，包括Plat、Pdcd5、Rnd3、Sirt1、Plaur、Runx1、Ccr1、Marveld1、Lmnb2和Chd7。我们发现低温治疗改变了TBI诱导的大鼠海马体m6A甲基化图谱的某些方面。
