Abdollahi Ahmad, Farsad-Akhtar Nader, Mohajel Kazemi Elham, Kolahi Maryam
Department of Plant, Cell and Molecular Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Physiol Mol Biol Plants. 2023 Feb;29(2):173-184. doi: 10.1007/s12298-023-01281-0. Epub 2023 Feb 3.
L., is a sprawling, leafy herb that grows in open, disturbed areas, known as St. John's wort, has a variety of secondary metabolites that can be used for medicinal and therapeutic purposes. Heavy metals have become the most dangerous pollutants in the environment. The effect of cadmium chloride, lead nitrate, silver nitrate, methyl jasmonate, and salicylic acid was studied on several morphometric and biochemical features of St. John's wort simultaneously using the Taguchi statistical method. The results showed cadmium chloride and lead nitrate reduced the morphometric and biochemical properties of St. John's wort while salicylic acid compensated for the adverse effects of heavy metals. Simultaneously, use of salicylic acid and silver nitrate with cadmium chloride and lead nitrate reduced the toxic effects of these metals on morphometric properties. Methyl jasmonate improved growth characteristics at low levels and inhibited at higher levels. Also, according to the results, salicylic acid could reduce the effects of heavy metals on the biochemical properties, while silver nitrate acts like heavy metals, especially at higher levels. Salicylic acid reduced the harmful effects of these heavy metals and at all levels was able to create a better induction effect on St. John's wort. These elicitors mainly changed the adverse effects of heavy metals by strengthening the pathways of the antioxidant system in St. John's wort. The research assumptions were validated, which suggests that the Taguchi method could be considered in an optimum culture of medicinal plants under different treatments such as heavy metals and elicitors.
贯叶连翘是一种蔓生的多叶草本植物，生长在开阔、受干扰的地区，它有多种次生代谢产物可用于医药和治疗目的。重金属已成为环境中最危险的污染物。采用田口统计方法，同时研究了氯化镉、硝酸铅、硝酸银、茉莉酸甲酯和水杨酸对贯叶连翘若干形态特征和生化特性的影响。结果表明，氯化镉和硝酸铅降低了贯叶连翘的形态特征和生化特性，而水杨酸则弥补了重金属的不利影响。同时，将水杨酸和硝酸银与氯化镉和硝酸铅一起使用，降低了这些金属对形态特征的毒性作用。茉莉酸甲酯在低浓度时改善生长特性，在高浓度时抑制生长特性。此外，根据结果，水杨酸可以降低重金属对生化特性的影响，而硝酸银的作用类似于重金属，尤其是在高浓度时。水杨酸降低了这些重金属的有害影响，并且在所有浓度下都能够对贯叶连翘产生更好的诱导作用。这些诱导剂主要通过加强贯叶连翘抗氧化系统的途径来改变重金属的不利影响。研究假设得到了验证，这表明在重金属和诱导剂等不同处理下的药用植物优化培养中，可以考虑使用田口方法。
