Ramesha C S, Soter N, Pickett W C
Inflammation and Immunology Research Department, Lederle Laboratories, American Cyanamid Company, Pearl River, New York 10965.
Agents Actions. 1987 Aug;21(3-4):382-3. doi: 10.1007/BF01966522.
Platelet activating factor was isolated from scales of psoriatic patients by the procedure of Bligh and Dyer and purified by silica gel thin layer chromatography. The purified PAF was digested with phospholipase C and the resulting diglyceride was derivatized into PFB ethers. The PAF-PFB ethers were analyzed using fused silica capillary chromatography-negative ion chemical ionization mass spectrometry. Different molecular species of PAF were identified by their negative ion mass spectra and by their elution time from the capillary column. All the molecular species had high abdundance (greater than 90%) of the molecular anion. 1-0-Hexadecyl-2-acetyl-GPC (16:0) was the major PAF species representing 51% of the total PAF. 17:0 and 18:1 were the next abundant species representing 15 and 16%, respectively. Several minor PAF molecular species were also present. The amount of each PAF molecular species was quantitated from 1-0-hexadecyl-2-2H3 acetyl-GPC used as the internal standard. Nanogram quantities of PAF were recovered from 100 mg of psoriatic scales. Significant amounts of lysoPAF were also present in these scales. The alkyl chain of the lysoPAF was compared with that of PAF.
采用布莱和戴尔的方法从银屑病患者的鳞屑中分离出血小板活化因子，并通过硅胶薄层色谱法进行纯化。将纯化后的血小板活化因子用磷脂酶C消化，所得甘油二酯衍生化为五氟苄基醚。使用熔融石英毛细管色谱 - 负离子化学电离质谱法分析血小板活化因子 - 五氟苄基醚。通过负离子质谱及其从毛细管柱的洗脱时间鉴定不同分子种类的血小板活化因子。所有分子种类的分子阴离子丰度都很高（大于90%）。1-0-十六烷基-2-乙酰基-GPC（16:0）是主要的血小板活化因子种类，占总血小板活化因子的51%。17:0和18:1是其次丰富的种类，分别占15%和16%。还存在几种次要的血小板活化因子分子种类。以1-0-十六烷基-2-2H3乙酰基-GPC作为内标对每种血小板活化因子分子种类的量进行定量。从100毫克银屑病鳞屑中回收了纳克量的血小板活化因子。这些鳞屑中也存在大量的溶血血小板活化因子。比较了溶血血小板活化因子和血小板活化因子的烷基链。
