Ramesha C S, Pickett W C
J Biol Chem. 1986 Jun 15;261(17):7592-5.
Rat peripheral or elicited polymorphonuclear leukocytes 90% deficient in arachidonic acid incorporate, after stimulation with the calcium ionophore A23187, 86% less acetate into platelet-activating factor than control. The total amount of platelet-activating factor in the ionophore stimulated elicited polymorphonuclear leukocytes deficient in arachidonate, measured by gas chromatography-negative ion chemical ionization mass spectrometry, was 84% less than that of control. The mass spectrometry also revealed the presence of various molecular species of platelet-activating factor ranging from 1-O-tetradecyl to 1-O-nonadecyl forms in both the deficient and control cells. However, the 1-O-hexadecyl was the predominate molecular species representing 79 and 96% of the total platelet-activating factor in the respective deficient and control cells. Other molecular species were less than 1.5 and 8.5% of the total for control and deficient polymorphonuclear leukocytes, respectively. Leukotriene B4 formation was also inhibited by 90% in the deficient cells. Both platelet-activating factor and leukotriene B4 biosynthesis could be partially restored in arachidonic acid-deficient cells by prelabeling the cells with arachidonate. This represents the first dietary link with platelet-activating factor biosynthesis.
花生四烯酸缺乏90%的大鼠外周或诱导多形核白细胞，在用钙离子载体A23187刺激后，与对照组相比，掺入血小板活化因子中的乙酸盐减少了86%。通过气相色谱 - 负离子化学电离质谱法测量，在离子载体刺激的花生四烯酸缺乏的诱导多形核白细胞中，血小板活化因子的总量比对照组少84%。质谱分析还显示，在缺乏组和对照组细胞中均存在从1 - O - 十四烷基到1 - O - 十九烷基形式的各种血小板活化因子分子种类。然而，1 - O - 十六烷基是主要的分子种类，在各自的缺乏组和对照组细胞中分别占血小板活化因子总量的79%和96%。其他分子种类在对照组和缺乏组多形核白细胞中分别占总量的不到1.5%和8.5%。白三烯B4的形成在缺乏组细胞中也被抑制了90%。通过用花生四烯酸盐预标记细胞，花生四烯酸缺乏的细胞中血小板活化因子和白三烯B4的生物合成均可部分恢复。这代表了与血小板活化因子生物合成的首个饮食关联。
