Shatarat A T, Halaiqah S H, Altarawneh I A, Smadi Z S, Badran D H, Al-Essa Mohamed K, Mohammed F I
Department of Anatomy and Histology /School of Medicine / University of Jordan; Faculty of Medicine, Aqaba Medical Sciences University, Aqaba, Jordan.
Department of Anatomy and Histology /School of Medicine / University of Jordan, Aqaba, Jordan.
Niger J Clin Pract. 2023 Feb;26(2):187-193. doi: 10.4103/njcp.njcp_397_22.
The leaves of Origanum are widely used in herbal medicine hence of having many beneficial ingredients, one of these important compounds is Carvacrol. The inhibitory effect of Carvacrol was the core of this study by applying different kinds of stimulants to smooth muscles in the wall of thoracic aorta in rats.
To investigate the pharmacological effects of Carvacrol, the main active ingredient present in the medicinal plant Origanum, on the contractile activity and morphology of the smooth muscle of the rat thoracic aorta.
After the thoracic aorta arteries were isolated and prepared for the experiments, each thoracic aorta was cut into 5-mm ring segments; different stimulants were used (Potassium Chloride, Norepinephrine, U46619, and α,β-methylene ATP) in the presence and absence of Carvacrol on four groups of rats. The isolated rings were placed and connected to a force transducer which in turn linked to a data acquisition system via an amplifier to record the effect of each stimulant. GraphPad Prism version 5.02 for Windows, one-way analysis of variance followed by Dunnett's multiple comparison test.
It was found out that Carvacrol obstructs the contractile responses elicited by exogenous NA, KCl, U46619, and α,β-methylene ATP in a concentration dependent manner.
The addition of Carvacrol in the experimental rats showed an increase in the thickness of tunica media as evident by the number of smooth muscle layers and laminae of elastic fibers. It was found that Carvacrol reduced the vascular smooth muscle contractility in the rat thoracic aorta. The mechanism of action is presumed to be achieved through interfering with the mobilization of both intracellular and extracellular Ca2 through different receptors. Furthermore, it might be suggested that Carvacrol in high doses stimulates smooth muscles in the wall of aorta leading to an increase in the thickness of tunica media layer.
牛至叶在草药医学中广泛应用，含有多种有益成分，其中重要的化合物之一是香芹酚。本研究的核心是通过对大鼠胸主动脉壁平滑肌施加不同刺激物来研究香芹酚的抑制作用。
研究药用植物牛至中主要活性成分香芹酚对大鼠胸主动脉平滑肌收缩活性和形态的药理作用。
分离并准备好胸主动脉用于实验后，将每条胸主动脉切成5毫米的环段；在有和没有香芹酚的情况下，对四组大鼠使用不同的刺激物（氯化钾、去甲肾上腺素、U46619和α,β-亚甲基ATP）。将分离的环段放置并连接到力传感器，力传感器再通过放大器连接到数据采集系统，以记录每种刺激物的作用。使用Windows版GraphPad Prism 5.02软件，采用单因素方差分析，随后进行Dunnett多重比较检验。
发现香芹酚以浓度依赖性方式阻碍外源性去甲肾上腺素、氯化钾、U46619和α,β-亚甲基ATP引起的收缩反应。
在实验大鼠中添加香芹酚后，中膜厚度增加，这可通过平滑肌层数量和弹性纤维层明显看出。发现香芹酚可降低大鼠胸主动脉血管平滑肌的收缩性。推测其作用机制是通过不同受体干扰细胞内和细胞外钙离子的动员来实现的。此外，可能提示高剂量的香芹酚会刺激主动脉壁的平滑肌，导致中膜层厚度增加。
