Carbohydrate Laboratory, School of Food Science and Engineering, South China University of Technology, 381 Wushan Rd., Tianhe District, Guangzhou 510640, PR China.
Carbohydrate Laboratory, School of Food Science and Engineering, South China University of Technology, 381 Wushan Rd., Tianhe District, Guangzhou 510640, PR China.
Carbohydr Polym. 2023 Jan 1;299:120146. doi: 10.1016/j.carbpol.2022.120146. Epub 2022 Sep 29.
A new micellization method was applied to produce the nano octenyl succinic anhydride (OSA) modified starch micelles with controllable size. The underlying mechanism was explored by using Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), dynamic light scattering (DLS), zeta-potential, surface tension, fluorescence spectra and transmission electron microscope (TEM). Due to the new starch modification method, the electrostatic repulsion between the deprotonation carboxyl groups prevented the aggregation of starch chains. With the progress of protonation, the weaken electrostatic repulsion and enhanced hydrophobic interaction driven the self-assembly of micelles. The size of micelles increased gradually with the increase of the protonation degree (PD) and concentration of OSA starch. However, a V-shaped trends were observed in the size as the increase of substitution of degree (DS). Curcuma loading test indicated that micelles had good encapsulated capability and the maximum value was 52.2 μg/mg. The understanding of the self-assembly behavior of OSA starch micelles can facilitate and improve the starch-based carrier designs used to synthesis complex and smart micelle delivery system with good biocompatibility.
一种新的胶束化方法被应用于制备具有可控尺寸的纳米辛烯基琥珀酸酐（OSA）改性淀粉胶束。通过使用傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）、动态光散射（DLS）、Zeta 电位、表面张力、荧光光谱和透射电子显微镜（TEM）来探索其潜在机制。由于新的淀粉改性方法，脱质子羧基之间的静电排斥阻止了淀粉链的聚集。随着质子化的进行，静电排斥的减弱和疏水性相互作用的增强驱动了胶束的自组装。随着质子化程度（PD）和 OSA 淀粉浓度的增加，胶束的尺寸逐渐增大。然而，在取代度（DS）增加的情况下，观察到了一个 V 形趋势。姜黄素负载测试表明，胶束具有良好的包封能力，最大值为 52.2μg/mg。对 OSA 淀粉胶束自组装行为的了解可以促进和改进基于淀粉的载体设计，用于合成具有良好生物相容性的复杂和智能胶束递药系统。
