Capetti Caio Cesar de Mello, Pellegrini Vanessa Oliveira Arnoldi, Espirito Santo Melissa Cristina, Cortez Anelyse Abreu, Falvo Maurício, Curvelo Antonio Aprigio da Silva, Campos Eleonora, Filgueiras Jefferson Gonçalves, Guimaraes Francisco Eduardo Gontijo, de Azevedo Eduardo Ribeiro, Polikarpov Igor
Instituto de Física de São Carlos, Universidade de São Paulo, Avenida Trabalhador São-carlense 400, 13566-590 São Carlos, SP, Brazil.
Instituto de Química de São Carlos, Universidade de São Paulo, Avenida Trabalhador São-carlense 400, 13566-590 São Carlos, SP, Brazil.
Carbohydr Polym. 2023 Jan 1;299:120174. doi: 10.1016/j.carbpol.2022.120174. Epub 2022 Sep 30.
Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via "green" and sustainable XOS production.
玉米芯是富含木聚糖的丰富农业废弃物。在此，我们使用一组来自GH10和GH11家族的重组内切和外切作用酶，比较了通过两种不同预处理途径（碱处理和水热法）获得的玉米芯木寡糖产量，这些酶对木聚糖取代具有不同的限制。此外，还评估了预处理对玉米芯样品化学成分和物理结构的影响。我们证明，碱预处理途径每克初始生物质可产生59毫克木寡糖，而通过使用GH10和GH11酶组合的水热预处理，木寡糖的总产率达到115毫克/克。这些结果有望通过“绿色”和可持续的木寡糖生产实现玉米芯的生态可持续酶法增值。
