The MOE Key Laboratory for Standardization of Chinese Medicines, SATCM Key Laboratory for New Resources, Quality Evaluation of Chinese Medicines, Institute of Chinese Mate ria Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, PR China.
School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, PR China.
Carbohydr Polym. 2023 Jan 1;299:120216. doi: 10.1016/j.carbpol.2022.120216. Epub 2022 Oct 14.
An arabinogalactan (PTPS-1-2) was isolated and characterized from Pollen Typhae, and its potential antitumor effects on activating macrophages to produce immunomodulatory factors and promoting apoptosis in colorectal cancer cells were investigated. Structural characterization showed that PTPS-1-2 had a molecular weight of 59 kDa and was composed of rhamnose, arabinose, glucuronic acid, galactose, and galacturonic acid with a molar ratio of 7.6: 17.1: 6.5: 61.4: 7.4. Its backbone was predominantly composed of T-β-D-Galp, 1,3-β-D-Galp, 1,6-β-D-Galp, 1,3,6-β-D-Galp, 1,4-α-D-GalpA, 1,2-α-L-Rhap, additionally, branches contained 1,5-α-L-Araf, T-α-L-Araf, T-β-D-4-OMe-GlcpA, T-β-D-GlcpA and T-α-L-Rhap. PTPS-1-2 activated RAW264.7 cell by triggering the NF-kB signaling pathway and M1 macrophage polarization. Furthermore, the conditioned medium (CM) of Mφ pretreated with PTPS-1-2 exerted marked antitumor effects by inhibiting RKO cell proliferation and suppressing cell colony formation. Collectively, our findings suggested that PTPS-1-2 might be a therapeutic option for the prevention and treatment of tumors.
从蒲黄中分离得到一种阿拉伯半乳聚糖（PTPS-1-2），并对其激活巨噬细胞产生免疫调节因子和促进结直肠癌细胞凋亡的潜在抗肿瘤作用进行了研究。结构表征表明，PTPS-1-2 的分子量为 59 kDa，由鼠李糖、阿拉伯糖、葡萄糖醛酸、半乳糖和半乳糖醛酸组成，摩尔比为 7.6:17.1:6.5:61.4:7.4。其骨架主要由 T-β-D-Galp、1,3-β-D-Galp、1,6-β-D-Galp、1,3,6-β-D-Galp、1,4-α-D-GalpA、1,2-α-L-Rhap 组成，此外，支链含有 1,5-α-L-Araf、T-α-L-Araf、T-β-D-4-OMe-GlcpA、T-β-D-GlcpA 和 T-α-L-Rhap。PTPS-1-2 通过触发 NF-κB 信号通路和 M1 巨噬细胞极化来激活 RAW264.7 细胞。此外，用 PTPS-1-2 预处理的 Mφ 的条件培养基（CM）通过抑制 RKO 细胞增殖和抑制细胞集落形成来发挥显著的抗肿瘤作用。总之，我们的研究结果表明，PTPS-1-2 可能是预防和治疗肿瘤的一种治疗选择。
