School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technology Research Center, Guangzhou 510641, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technology Research Center, Guangzhou 510641, China.
Int J Biol Macromol. 2024 Nov;279(Pt 3):134616. doi: 10.1016/j.ijbiomac.2024.134616. Epub 2024 Aug 8.
Arabinogalactan exhibits many biological activities, which is the candidate for functional food ingredients. However, there is limited research on the arabinogalactan from Moringa Oleifera leaf, and its structure needs to be more accurately characterized. This study investigated structural characteristics and immunomodulatory activity of a high-purity polysaccharide from Moringa oleifera leaf (i.e. MOLP-PE) to further explore arabinogalactan from Moringa Oleifera leaf and its potential application area. The results showed that MOLP-PE was a unique type II arabinogalactan: the main chain consisted of → 3, 4)-α-D-Galp-(1→, →3)-β-D-Galp-(1→ and →2, 4)-β-D-Rhap-(1→, with branches at the C-4 position of →3, 4)-α-D-Galp-(1→ and →2, 4)-β-D-Rhap-(1→, consisting of →5)-α-L-Araf-(1→, →3)-α-L-Araf-(1→, →6)-β-D-Galp-(1→ and →4)-β-D-GalpA-(1→. Compared with arabinogalactan from larch, galactan and arabinan, MOLP-PE exhibited stronger ability in stimulating proliferation, phagocytosis and cytokines release of macrophages and bound with Toll-like receptor 4 closer via more binding sites, which might be due to its higher contents of 1,3-linked-Galp and 1,5-linked-Araf. These findings elucidated that MOLP-PE, as type II arabinogalactan with a unique structure, could be exploited as an immunomodulatory food ingredient.
阿拉伯半乳聚糖具有多种生物活性，是功能食品成分的候选物。然而，关于辣木叶阿拉伯半乳聚糖的研究有限，其结构需要更准确地表征。本研究调查了一种高纯度辣木叶多糖（即 MOLP-PE）的结构特征和免疫调节活性，以进一步探索辣木叶阿拉伯半乳聚糖及其潜在应用领域。结果表明，MOLP-PE 是一种独特的 II 型阿拉伯半乳聚糖：主链由 →3,4)-α-D-Galp-(1→、→3)-β-D-Galp-(1→和 →2,4)-β-D-Rhap-(1→组成，支链位于 →3,4)-α-D-Galp-(1→和 →2,4)-β-D-Rhap-(1→的 C-4 位，由 →5)-α-L-Araf-(1→、→3)-α-L-Araf-(1→、→6)-β-D-Galp-(1→和 →4)-β-D-GalpA-(1→组成。与落叶松阿拉伯半乳聚糖、半乳糖和阿拉伯聚糖相比，MOLP-PE 具有更强的刺激巨噬细胞增殖、吞噬和细胞因子释放的能力，并且通过更多的结合位点与 Toll 样受体 4 结合更紧密，这可能是由于其 1,3-连接的 Galp 和 1,5-连接的 Araf 含量较高。这些发现表明，MOLP-PE 作为一种具有独特结构的 II 型阿拉伯半乳聚糖，可作为一种免疫调节的功能性食品成分。
