Lai Peng, Yang Xin, Li Yun-Hui, Yin Yan-Ling, Yao Qian, Huang Shuang, Fan Ying-Ying, Song Jun-Ke, Zhao Guang-Hui
Key Laboratory of Ruminant Disease Prevention and Control (West), College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
Parasitol Res. 2023 Apr;122(4):989-996. doi: 10.1007/s00436-023-07803-9. Epub 2023 Mar 7.
Cryptosporidium parvum is an important apicomplexan parasite causing severe diarrhea in both humans and animals. Calmodulin (CaM), a multifunctional and universal calcium-binding protein, contributes to the growth and development of apicomplexan parasites, but the role of CaM in C. parvum remains unknown. In this study, the CaM of C. parvum encoded by the cgd2_810 gene was expressed in Escherichia coli, and the biological functions of CpCaM were preliminarily investigated. The transcriptional level of the cgd2_810 gene peaked at 36 h post infection (pi), and the CpCaM protein was mainly located around the nucleus of the whole oocysts, in the middle of sporozoites and around the nucleus of merozoites. Anti-CpCaM antibody reduced the invasion of C. parvum sporozoites by 30.69%. The present study indicates that CpCaM is potentially involved in the growth of C. parvum. Results of the study expand our knowledge on the interaction between host and Cryptosporidium.
微小隐孢子虫是一种重要的顶复门寄生虫，可导致人和动物严重腹泻。钙调蛋白（CaM）是一种多功能通用钙结合蛋白，对顶复门寄生虫的生长和发育有作用，但CaM在微小隐孢子虫中的作用尚不清楚。本研究中，由cgd2_810基因编码的微小隐孢子虫CaM在大肠杆菌中表达，并对CpCaM的生物学功能进行了初步研究。cgd2_810基因的转录水平在感染后36小时达到峰值，CpCaM蛋白主要位于整个卵囊的细胞核周围、子孢子中部和裂殖子细胞核周围。抗CpCaM抗体使微小隐孢子虫子孢子的侵袭减少了30.69%。本研究表明CpCaM可能参与微小隐孢子虫的生长。该研究结果扩展了我们对宿主与隐孢子虫之间相互作用的认识。
