Kale Ashish, Kale Ashwini
Department of Obstetrics and Gynaecology, Ashakiran Hospitals and ASHA IVF Centre, #555, Narayan Chambers, Opposite Narayan Peth Police Station, Pune, Maharashtra 411030 India.
J Obstet Gynaecol India. 2023 Feb;73(1):57-61. doi: 10.1007/s13224-022-01703-1. Epub 2022 Oct 21.
To evaluate the oocyte retrieval rate and blastocyst formation rate with DuoStim protocol in patients belonging to POSEIDON groups 3 and 4.
This observational, retrospective, single-center study including 90 patients belonging to POSEIDON groups 3 and 4 was conducted at a tertiary care hospital from October 2017 to March 2020. Patients were allocated into two groups based on POSEIDON classification criteria: group A (POSEIDON group 3) and group B (POSEIDON group 4). DuoStim protocol was performed with human menopausal gonadotropin (hMG) at 225 IU and 300 IU in groups A and B, respectively. Study groups were again subdivided by considering the phase in which stimulation had been done [follicular phase stimulation (FPS) and luteal phase stimulation (LPS)], and then, inference was made accordingly in terms of oocytes retrieval rate and blastocysts formation rate. Data were compiled and analyzed using statistical software SPSS version 20.
The baseline characteristics of two groups were compatible with POSEIDON groups 3 and 4. A significant difference was found between study groups with respect to age and anti-mullerian hormone levels ( < 0.05). Significantly, a greater number of oocytes and blastocysts were obtained in LPS stage, substantially more in group A (3.69 ± 3.4 vs. 4.52 ± 4.3 and 1.36 ± 0.65 vs. 3.17 ± 1.84) than group B (2.2 ± 1.36 vs. 3.6 ± 4.5 and 0.41 ± 0.8 vs. 1.29 ± 2.04). A greater blastulation rate (50 vs. 66.7% and 33.3 vs. 50%) and 100% oocyte maturity rate were observed in LPS stage of both the study groups.
In patients belonging to POSEIDON groups 3 and 4, the number of oocytes retrieved and blastocyst formation rate were greater in LPS stage when compared to FPS with DuoStim protocol.
评估在POSEIDON 3组和4组患者中使用双刺激方案的卵母细胞采集率和囊胚形成率。
这项观察性、回顾性、单中心研究于2017年10月至2020年3月在一家三级护理医院进行，纳入了90例POSEIDON 3组和4组的患者。根据POSEIDON分类标准将患者分为两组：A组（POSEIDON 3组）和B组（POSEIDON 4组）。A组和B组分别使用225IU和300IU的人绝经期促性腺激素（hMG）进行双刺激方案。研究组再根据刺激进行的阶段[卵泡期刺激（FPS）和黄体期刺激（LPS）]进一步细分，然后据此推断卵母细胞采集率和囊胚形成率。使用统计软件SPSS 20版对数据进行整理和分析。
两组的基线特征与POSEIDON 3组和4组相符。研究组在年龄和抗苗勒管激素水平方面存在显著差异（<0.05）。值得注意的是，在LPS阶段获得的卵母细胞和囊胚数量更多，A组（3.69±3.4对4.52±4.3以及1.36±0.65对3.17±1.84）明显多于B组（2.2±1.36对3.6±4.5以及0.41±0.8对1.29±2.04）。在两个研究组的LPS阶段均观察到更高的囊胚形成率（50%对66.7%以及33.3%对50%）和100%的卵母细胞成熟率。
在POSEIDON 3组和4组患者中，与双刺激方案的FPS相比，LPS阶段采集的卵母细胞数量和囊胚形成率更高。
