Chemistry Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi Arabia.
Chemistry Department, Faculty of Science, University of Jeddah, Jeddah, Saudi Arabia.
Luminescence. 2023 Apr;38(4):477-486. doi: 10.1002/bio.4472. Epub 2023 Mar 14.
A sensitive and selective phenothiazine-based sensor (PTZ) has been successfully synthesized. The sensor PTZ displayed specific identification of CN 'turn-off' fluorescence responses with a quick reaction and strong reversibility in an acetonitrile:water (90:10, V/V) solution. The sensor PTZ for detecting CN exhibits the marked advantages of quenching the fluorescence intensity, fast response time (60 s), and low value of the detection limit. The concentration that is authorized for drinking water by the WHO (1.9 μM) is far higher than the detection limit, which was found to be 9.11 × 10 . The sensor displays distinct colorimetric and spectrofluorometric detection for CN anion due to the addition of CN anion to the electron-deficient vinyl group of PTZ, which reduces intramolecular charge transfer efficiencies. The 1:2 binding mechanism of PTZ with CN was validated by fluorescence titration, Job's plot, HRMS, H NMR, FTIR analysis, and density functional theory (DFT) investigations, among other methods. Additionally, the PTZ sensor was successfully used to precisely and accurately detect cyanide anions in actual water samples.
一种灵敏且选择性的基于吩噻嗪的传感器（PTZ）已经被成功合成。传感器 PTZ 在乙腈：水（90:10，V/V）溶液中显示出对 CN 的特定识别“关闭”荧光响应，具有快速反应和强可逆性。用于检测 CN 的传感器 PTZ 具有荧光强度猝灭、快速响应时间（60s）和低检测限的显著优点。世界卫生组织（WHO）授权的饮用水浓度（1.9μM）远高于检测限，检测限为 9.11×10-6M。由于 CN 阴离子的加入，降低了 PTZ 的缺电子乙烯基的分子内电荷转移效率，因此传感器对 CN 阴离子表现出明显的比色和荧光检测。通过荧光滴定、Job 图、高分辨率质谱（HRMS）、1H NMR、傅里叶变换红外（FTIR）分析和密度泛函理论（DFT）研究等方法验证了 PTZ 与 CN 的 1:2 结合机制。此外，PTZ 传感器还成功用于精确和准确地检测实际水样中的氰化物阴离子。
