Wen Cuiping, Zhang Zhong, Shi Qianqian, Niu Runzi, Duan Xiaoshan, Shen Bingqi, Li Xingang
College of Forestry, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518116, China.
J Agric Food Chem. 2023 Mar 22;71(11):4599-4614. doi: 10.1021/acs.jafc.2c08679. Epub 2023 Mar 7.
Jujube ( Mill.) is rich in valuable bioactive triterpenoids. However, the regulatory mechanism underlying triterpenoid biosynthesis in jujube remains poorly studied. Here, we characterized the triterpenoid content in wild jujube and cultivated jujube. The triterpenoid content was higher in wild jujube than in cultivated jujube, triterpenoids were most abundant in young leaves, buds, and later stages of development. The transcriptome analysis and correlation analysis showed that differentially expressed genes (DEGs) were enriched in the terpenoid synthesis pathways, and triterpenoids content was strongly correlated with farnesyl diphosphate synthase (), squalene synthase (), and transcription factors and expression. Gene overexpression and silencing analysis indicated that and were key genes in triterpenoid biosynthesis and transcription factors and regulated triterpenoid biosynthesis. Subcellular localization experiments showed that and were localized to the nucleus and endoplasmic reticulum and and were localized to the nucleus. Yeast one-hybrid, glucuronidase activity, and dual-luciferase activity assays suggested that and regulate triterpenoid biosynthesis by directly binding and activating the promoters of and . These findings provide insights into the underlying regulatory network of triterpenoids metabolism in jujube and lay theoretical and practical foundation for molecular breeding.
枣（Mill.）富含珍贵的生物活性三萜类化合物。然而，枣中三萜类化合物生物合成的调控机制仍研究不足。在此，我们对野生枣和栽培枣中的三萜类化合物含量进行了表征。野生枣中的三萜类化合物含量高于栽培枣，三萜类化合物在幼叶、芽和发育后期最为丰富。转录组分析和相关性分析表明，差异表达基因（DEGs）在萜类合成途径中富集，并且三萜类化合物含量与法尼基二磷酸合酶（）、角鲨烯合酶（）以及转录因子和的表达密切相关。基因过表达和沉默分析表明，和是三萜类化合物生物合成中的关键基因，转录因子和调控三萜类化合物的生物合成。亚细胞定位实验表明，和定位于细胞核和内质网，和定位于细胞核。酵母单杂交、葡萄糖醛酸酶活性和双荧光素酶活性测定表明，和通过直接结合并激活和的启动子来调控三萜类化合物的生物合成。这些发现为枣中三萜类化合物代谢的潜在调控网络提供了见解，并为分子育种奠定了理论和实践基础。
需注意，原文中部分括号内容缺失具体信息，翻译时保留了括号形式。
