Chen Naiying, Yao Sicheng, Li Mingming, Wang Qiuyue, Sun Xinxing, Feng Xun, Chen Yang
Department of Pharmaceutics, School of Pharmacy, China Medical University, No.77 Puhe Road, Shenyang North New Area, Shenyang 110122, China.
Department of Sanitary Chemistry, School of Public Health, Shenyang Medical College, No.146 Yellow River North Street, Shenyang 110034, China.
Biomacromolecules. 2023 Apr 10;24(4):1648-1661. doi: 10.1021/acs.biomac.2c01431. Epub 2023 Mar 7.
The use of polydopamine-based bioinspired nanomaterials has shed new light on advanced drug delivery arising from their efficient surface functionalization. More recently, the polydopamine self-assemblies formed in two different modalities, i.e., nonporous and mesoporous nanoparticles, have begun to attract attention due to their expedient and versatile properties. However, their possibility for use in dermal drug delivery for local therapy, as well as their interaction with the skin, has not yet been demonstrated. Our study aimed to compare and explore the feasibility of the self-assembled nonporous polydopamine nanoparticles (PDA) and mesoporous polydopamine nanoparticles (mPDA) for local skin drug delivery. The formation of the PDA and mPDA structures was confirmed by the UV-vis-NIR absorption spectrum, the Fourier transform infrared spectroscopy, and the nitrogen adsorption/desorption isotherms. Using retinoic acid (RA) as the model drug, their effects on drug loading, release, photostability, skin penetration, and radical scavenging were investigated. Laser scanning confocal microscopy (LSCM) and hematoxylin and eosin (H&E) were introduced to probe their delivery routes and possible interaction with the skin. The results indicated that both PDA and mPDA could reduce the photodegradation of RA, and mPDA showed significantly better radical scavenging activity and drug loading capacity. The permeation study revealed that both PDA and mPDA significantly enhanced the delivery of RA into the deep skin layers by comparison with the RA solution, in which follicular and intercellular pathways existed, and alteration in the structure of stratum corneum was observed. In light of drug loading capacity, size controllability, physical stability, as well as radical scavenging activity, mPDA was more preferable due to the improvement of these factors. This work demonstrated the feasibility and promising application of PDA and mPDA nanoparticles for dermal drug delivery, and the comparative concept of these two types of biomaterials can provide implications for their use in other fields.
基于聚多巴胺的仿生纳米材料因其高效的表面功能化，为先进药物递送带来了新的曙光。最近，以两种不同形态形成的聚多巴胺自组装体，即无孔和介孔纳米颗粒，因其便捷且多样的性质开始受到关注。然而，它们在用于局部治疗的皮肤药物递送中的可能性，以及它们与皮肤的相互作用，尚未得到证实。我们的研究旨在比较和探索自组装无孔聚多巴胺纳米颗粒（PDA）和介孔聚多巴胺纳米颗粒（mPDA）用于局部皮肤药物递送的可行性。通过紫外 - 可见 - 近红外吸收光谱、傅里叶变换红外光谱和氮吸附/解吸等温线证实了PDA和mPDA结构的形成。以维甲酸（RA）作为模型药物，研究了它们对药物负载、释放、光稳定性、皮肤渗透和自由基清除的影响。引入激光扫描共聚焦显微镜（LSCM）和苏木精和伊红（H&E）染色来探究它们的递送途径以及与皮肤可能的相互作用。结果表明，PDA和mPDA都能减少RA的光降解，并且mPDA表现出明显更好的自由基清除活性和药物负载能力。渗透研究表明，与RA溶液相比，PDA和mPDA都显著增强了RA向皮肤深层的递送，其中存在毛囊和细胞间途径，并且观察到角质层结构的改变。鉴于药物负载能力、尺寸可控性、物理稳定性以及自由基清除活性，由于这些因素的改善，mPDA更具优势。这项工作证明了PDA和mPDA纳米颗粒用于皮肤药物递送的可行性和有前景的应用，并且这两种生物材料的比较概念可为它们在其他领域的应用提供启示。
