挥发性次生代谢组和转录组分析揭示了紫丁香和欧洲丁香香气生物合成的不同调控机制。

Volatile secondary metabolome and transcriptome analysis reveals distinct regulation mechanism of aroma biosynthesis in Syringa oblata and S. vulgaris.

作者信息

Yang Zhiying, Zhu Yuanyuan, Zhang Xu, Zhang Hailiang, Zhang Xiaoyu, Liu Genzhong, Zhao Qingzhu, Bao Zhilong, Ma Fangfang

机构信息

Weifang Academy of Agricultural Sciences, Weifang, 261071, Shandong, China.

State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China.

出版信息

Plant Physiol Biochem. 2023 Mar;196:965-973. doi: 10.1016/j.plaphy.2023.03.003. Epub 2023 Mar 2.

DOI:10.1016/j.plaphy.2023.03.003

PMID:36889235

Abstract

Lilacs have high ornamental value due to their strong aroma. However, the molecular regulatory mechanisms of aroma biosynthesis and metabolism in lilac were largely unclear. In this study, two varieties with distinct aroma, Syringa oblata 'Zi Kui' (faint aroma) and Syringa vulgaris 'Li Fei' (strong aroma), were used for exploring the regulation mechanism of aroma difference. Via GC-MS analysis, a total of 43 volatile components were identified. Terpene volatiles was the most abundant volatiles constituting the aroma of two varieties. Notably, 3 volatile secondary metabolites were unique in 'Zi Kui' and 30 volatile secondary metabolites were unique in 'Li Fei'. Then, a transcriptome analysis was performed to clarify the regulation mechanism of aroma metabolism difference between these two varieties, and identified 6411 differentially expressed genes (DEGs). Interestingly, ubiquinone and other terpenoid-quinone biosynthesis genes were significantly enriched in DEGs. We further conducted a correlation analysis between the volatile metabolome and transcriptome and found that TPS, GGPPS, and HMGS genes might be the key contributors to the differences in floral fragrance composition between the two lilac varieties. Our study improves the understanding in the regulation mechanism of Lilac aroma and would help improve the aroma of ornamental crops by metabolic engineering.

摘要

丁香花因其浓郁的香气而具有很高的观赏价值。然而，丁香花香气生物合成和代谢的分子调控机制在很大程度上尚不清楚。在本研究中，选用了两个香气不同的品种，紫丁香‘紫魁’（淡香）和西洋丁香‘李菲’（浓香），来探究香气差异的调控机制。通过气相色谱-质谱联用（GC-MS）分析，共鉴定出43种挥发性成分。萜类挥发物是构成两个品种香气的最主要挥发物。值得注意的是，有3种挥发性次生代谢产物在‘紫魁’中独特存在，30种挥发性次生代谢产物在‘李菲’中独特存在。随后，进行了转录组分析以阐明这两个品种间香气代谢差异的调控机制，并鉴定出6411个差异表达基因（DEGs）。有趣的是，泛醌和其他萜类醌生物合成基因在DEGs中显著富集。我们进一步对挥发性代谢组和转录组进行了相关性分析，发现TPS、GGPPS和HMGS基因可能是导致两个丁香品种花香成分差异的关键因素。我们的研究增进了对丁香香气调控机制的理解，并将有助于通过代谢工程改善观赏作物的香气。