Zhu Xiusheng, Huang Qitong, Luo Jing, Kong Dashuai, Zhang Yubo
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Livestock and Poultry Multi-omics of MARA, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Animal Breeding and Genomics, Wageningen University & Research, Wageningen 6708PB, the Netherlands.
Comput Struct Biotechnol J. 2023 Feb 16;21:1728-1737. doi: 10.1016/j.csbj.2023.02.028. eCollection 2023.
Gene regulatory networks are now at the forefront of precision biology, which can help researchers better understand how genes and regulatory elements interact to control cellular gene expression, offering a more promising molecular mechanism in biological research. Interactions between the genes and regulatory elements involve different promoters, enhancers, transcription factors, silencers, insulators, and long-range regulatory elements, which occur at a ∼10 µm nucleus in a spatiotemporal manner. In this way, three-dimensional chromatin conformation and structural biology are critical for interpreting the biological effects and the gene regulatory networks. In the review, we have briefly summarized the latest processes in three-dimensional chromatin conformation, microscopic imaging, and bioinformatics, and we have presented the outlook and future directions for these three aspects.
基因调控网络如今处于精准生物学的前沿，它能帮助研究人员更好地理解基因与调控元件如何相互作用以控制细胞基因表达，为生物学研究提供了更具前景的分子机制。基因与调控元件之间的相互作用涉及不同的启动子、增强子、转录因子、沉默子、绝缘子和长程调控元件，这些相互作用在约10微米的细胞核中以时空方式发生。通过这种方式，三维染色质构象和结构生物学对于解释生物学效应和基因调控网络至关重要。在这篇综述中，我们简要总结了三维染色质构象、显微成像和生物信息学的最新进展，并对这三个方面的前景和未来方向进行了展望。
