Phengchat Rinyaporn, Malac Marek, Hayashida Misa
Graduate School of Human Development and Environment, Kobe University, 3-11 Tsurukabuto, Nada-ku, Kobe, 657-8501, Japan.
Nanotechnology Research Centre, National Research of Council, 11421 Saskatchewan Drive, T6G 2 M9, Edmonton, Alberta, Canada.
Chromosome Res. 2021 Mar;29(1):63-80. doi: 10.1007/s10577-021-09661-6. Epub 2021 Mar 18.
Our understanding of the inner structure of metaphase chromosomes remains inconclusive despite intensive studies using multiple imaging techniques. Transmission electron microscopy has been extensively used to visualize chromosome ultrastructure. This review summarizes recent results obtained using two transmission electron microscopy-based techniques: electron tomography and electron diffraction. Electron tomography allows advanced three-dimensional imaging of chromosomes, while electron diffraction detects the presence of periodic structures within chromosomes. The combination of these two techniques provides results contributing to the understanding of local structural organization of chromatin fibers within chromosomes.
尽管使用多种成像技术进行了深入研究，但我们对中期染色体内部结构的理解仍无定论。透射电子显微镜已被广泛用于观察染色体超微结构。本综述总结了使用两种基于透射电子显微镜的技术获得的最新结果：电子断层扫描和电子衍射。电子断层扫描可对染色体进行先进的三维成像，而电子衍射则可检测染色体内部周期性结构的存在。这两种技术的结合为理解染色质纤维在染色体中的局部结构组织提供了帮助。
